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GF155 Epidermal Growth Factor Protein, Recombinant mouse

The Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal & epithelial cells.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
UniProt Number: P01133, P01132
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GF155
500 µg  
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      개요

      주요 사양표

      SourcePurity
      E.coliGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of EGF.
      Description
      Catalogue NumberGF155
      ReplacesGF123
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionEpidermal Growth Factor Protein, Recombinant mouse
      OverviewEpidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal and epithelial cells. Recombinant murine EGF is a 6.2 kDa protein containing 54 amino acid residues.
      Alternate Names
      • EGF
      Product Information
      PresentationLyophilized from sterile filtered 10 mM Sodium Phosphate, pH 7.5, containing no additives.
      Quality LevelMQ100
      Applications
      ApplicationThe Epidermal Growth Factor (EGF) is a polypeptide growth factor, which stimulates the proliferation of a wide range of epidermal & epithelial cells.
      Application NotesFor most in vitro applications, EGF exerts its biological activity in the concentration range of 0.5 to 25.0 ng/mL. Responding cells are (partial list): Epidermal mesothelial cells.
      Biological Information
      PurityGreater than 98% by SDS-PAGE. Endotoxin level is less than 0.1 ng per μg (1EU/ug) of EGF.
      SourceE.coli
      Specific ActivityMouse EGF is fully biologically active when compared to standards. The ED50, as determined by its dose-dependent mitogenic activity on BALB/c 3T3 cells, is ≤ 0.15 ng/mL, corresponding to a specific activity of ≥ 6.6 x 10<sup>6</sup> units/mg.
      Entrez Gene SummaryEpidermal growth factor has a profound effect on the differentiation of specific cells in vivo and is a potent mitogenic factor for a variety of cultured cells of both ectodermal and mesodermal origin. The EGF precursor is believed to exist as a membrane-bound molecule which is proteolytically cleaved to generate the 53-amino acid peptide hormone that stimulates cells to divide.
      Gene Symbol
      • EGF
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01133 # EGF stimulates the growth of various epidermal and epithelial tissues in vivo and in vitro and of some fibroblasts in cell culture.
      SIZE: 1207 amino acids; 133946 Da
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      SIMILARITY: SwissProt: P01133 ## Contains 9 EGF-like domains. & Contains 9 LDL-receptor class B repeats.
      Stem Cell Type
      • Neural Stem Cells
      • Epithelial Cells
      • Mesenchymal Stem Cells
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe lyophilized EGF, though stable at room temperature, is best stored desiccated at -20°C. Reconstitute with water to a concentration of 0.1 - 1 mg/mL. This solution can be diluted into other buffered solutions and stored at 4°C for 1 week or -20°C for future use. Reconstituted EGF should be maintained at -20°C in undiluted aliquots for up to one year.
      Packaging Information
      Material Size500 µg
      Global Trade Item Number
      카탈로그 번호 GTIN
      GF155 04053252676178