Millipore Sigma Vibrant Logo

324715 Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
324715
가격 및 재고 조회

개요

Replacement Information

가격 및 재고여부

카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
324715-5UGCN
재고 정보 검색...
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      		 Plastic ampoule 5 μg
      가격 검색...
      가격을 검색할 수 없습니다
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      가격 문의 추가 정보
      ()이 할인됨
       
      —
      가격 문의
      		 —
      Description
      OverviewNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK. Suggested working concentration: 1:20 to 1:100 (protease:protein by weight) for sequence analysis.
      Catalogue Number324715
      Brand Family Calbiochem®
      References
      ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
      Product Information
      CAS number72561-05-8
      Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-<i>p</i>NA per min at 25°C, pH 7.7.
      EC number3.4.21.50
      FormLyophilized
      FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥90% by SDS-PAGE
      Specific Activity≥200 units/mg protein
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      R PhraseR: 20-36/37/38-42/43

      Harmful by inhalation.
      Irritating to eyes, respiratory system and skin.
      May cause sensitization by inhalation and skin contact.
      S PhraseS: 22-26-36

      Do not breathe dust.
      In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
      Wear suitable protective clothing.
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Harmful
      Storage +2°C to +8°C
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      324715-5UGCN 04055977215960

      Documentation

      Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Endoproteinase Lys-C, Sequencing Grade, Lysobacter enzymogenes Certificates of Analysis

      TitleLot Number
      324715

      References

      참고문헌 보기
      Jekel, P.A., et al. 1983. Anal. Biochem. 134, 347.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision01-February-2012 RFH
      DescriptionNative endoproteinase Lys-C from Lysobacter enzymogenes. Serine protease that specifically hydrolyzes amide and peptide ester bonds at the carboxylic side of lysine in peptides and proteins. Designed for protein sequencing or sequence verification, analysis of protein structural domains, and cleavage of fusion proteins. Inhibited by aprotinin, DFP, leupeptin, and TLCK.
      FormLyophilized
      FormulationLyophilized from 50 mM HEPES, 10 mM EDTA, 5 mg/ml raffinose, pH 8.0.
      Recommended reaction conditions
      Protocol 1. Dissolve the proteins to be sequenced in 25 mM Tris-HCl, pH 8.5, and 1 mM EDTA. For proteins that are difficult to solubilize, add urea, SDS, or guanidine-HCl to the digestion buffer prior to solubilization. If urea is used, add 20 mmol/l of methylamine. 2. The suggested working concentration is 1:20-1:100 (protease:protein by weight). 3. Incubate for 2-18 h at 37°C.
      CAS number72561-05-8
      EC number3.4.21.50
      Purity≥90% by SDS-PAGE
      Specific activity≥200 units/mg protein
      Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Tos-Gly-Pro-Lys-pNA per min at 25°C, pH 7.7.
      SolubilityReconstitute in 50 µl of distilled H₂O.
      Storage +2°C to +8°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 2 days at 4°C or for up to 1 month at -20°C.
      Toxicity Harmful
      ReferencesJekel, P.A., et al. 1983. Anal. Biochem. 134, 347.

      관련 제품 및 어플리케이션

      Related Products

      카탈로그 번호 설명  
      164598 Aminopeptidase M, Porcine Kidney 가격 및 재고 조회
      324682 Elastase, Porcine Pancreas, High Purity, Crystallized 가격 및 재고 조회
      324708 Endoproteinase Asp-N, Excision Grade, Pseudomonas fragi 가격 및 재고 조회
      324713 Endoproteinase Glu-C, Excision Grade, Staphylococcus aureus 가격 및 재고 조회
      516360 Pepsin, Porcine Stomach Mucosa 가격 및 재고 조회

      카테고리

      Life Science Research > Proteins and Enzymes > Other Enzymes