1.51450
Chromolith® RP-18 endcapped (monolithic) HPLC Columns
L × I.D.50 mm x 4.6 mm HPLC Column
동의어(들):
Chromolith® SpeedROD RP-18 endcapped 50-4.6 HPLC column
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제품정보 (DICE 배송 시 비용 별도)
UNSPSC Code:
41115709
NACRES:
SB.52
Matrix active group:
C18 (RP18), C18 bonding phase
Pore size:
13 nm mesoporosity, 2 μm macroporosity
Matrix:
monolithic silica
제품 이름
Chromolith® RP-18 endcapped, L × I.D.50 mm x 4.6 mm HPLC column
material
PEEK column
Quality Segment
agency
suitable for USP L1
product line
Chromolith®
feature
endcapped
packaging
1 ea of
extent of labeling
18% Carbon loading
parameter
200 bar pressure, 45 °C max. temp.
technique(s)
HPLC: suitable, LC/MS: suitable, mass spectrometry (MS): suitable (High)
column L × I.D.
5 cm × 4.6 mm
matrix
monolithic silica
matrix active group
C18 (RP18), C18 bonding phase
pore size
13 nm mesoporosity, 2 μm macroporosity
operating pH range
2-7.5
separation technique
reversed phase
storage temp.
no temp limit
General description
Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.
Chromolith® SpeedROD RP-18 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® SpeedROD RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® SpeedROD RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for fast separations on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
Or
Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451]
Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Need more efficiency?
Chromolith® HighResolution RP-18 endcapped 50-4.6 HPLC columns [1.52021] provide a column efficiency exceeding 140,000 plates/meter.
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Chromolith® SpeedROD RP-18 endcapped columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® SpeedROD RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith® SpeedROD RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 50 mm length and 4.6 mm I.D. is the best choice for fast separations on any HPLC System.
The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.51452] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
Or
Chromolith® RP-18 endcapped 10-4.6 guard cartridges (3 pieces) [1.51451]
Guard Cartridge Holder 10 mm x 4.6 mm [1.52033]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]
Need more efficiency?
Chromolith® HighResolution RP-18 endcapped 50-4.6 HPLC columns [1.52021] provide a column efficiency exceeding 140,000 plates/meter.
Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns
All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument
Application
- Simultaneous determination of eight arginine-related metabolites in cellular extracts using liquid chromatography-tandem mass spectrometry.: This study explores the usage of the Chromolith® RP-18 endcapped column for precise metabolic profiling, showcasing its effectiveness in analyzing arginine-related metabolites with high resolution and throughput (Amatya et al., 2020).
- Rapid Determination of 30 Polyphenols in Tongmai Formula, a Combination of Puerariae Lobatae Radix, Salviae Miltiorrhizae Radix et Rhizoma, and Chuanxiong Rhizoma, via Liquid Chromatography-Tandem Mass Spectrometry.: The use of Chromolith® RP-18 endcapped allowed for the fast and efficient separation of polyphenols in complex herbal formulations, illustrating its capabilities in herbal chemistry applications (Wang et al., 2017).
- Simultaneous Liquid Chromatographic Determination of Ebastine with Two Sympathomimetic Drugs Using a Monolithic Column.: This research highlights the Chromolith® RP-18 endcapped column′s ability to simultaneously analyze multiple compounds, thereby providing a method that improves the efficiency of pharmaceutical analyses (Ibrahim and Wahba, 2017).
Analysis Note
Carbon content: 17.0 - 18.6 %
Pressure: ≤ 15 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.6 - 4.6
Tailing (USP): 0.90 - 1.55 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35
Pressure: ≤ 15 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.6 - 4.6
Tailing (USP): 0.90 - 1.55 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35
Legal Information
Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany
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