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Centricon® Plus-70 Centrifugal Filter Units

Large volume sample concentration

관련 자료

개요

  • 90% typical recovery
  • 50X to 200X concentration
  • Low hold-up volume
  • Polypropylene housing minimizes binding
  • Deadstop prevents spinning to dryness
  • Ready to use. Nothing to assemble

Centrifugal Filter Units

  • Concentrating and desalting chromatography column eluates
  • Concentrating monoclonal antibodies
  • Concentrating proteins or viruses from culture supernatants
  • Clarifying tissue homogenates and cell lysates
  • Buffer exchange or diafiltration

Typical Flow Rates

Ultracel PL-30 Membrane at 3,500 x g

Ultracel PL-30 Membrane at 3,500 x g

Typical Retentate (Concentrate) Recovery
Membrane NMWL Protein Solute Protein Retentate
10,000 Cytochrome c, 12.4 kDa
(0.25 mg/mL)		 94
30,000 Bovine Serum Albumin,
67 kDa (1 mg/mL)		 96
100,000 IgG, 156 kDa
(1 mg/mL)		 91

Concentrate samples in the 15 mL-70 mL volume range using Centricon® Plus-70 centrifugal filter units. The large deadstop volume of 350 µL prevents spinning samples to dryness. The Centricon® Plus-70 has two vertical regenerated cellulose membranes for fast, efficient tangential flow. The device is available with four different pore size membranes (3, 10, 30 and 100 kDa NMWL).

Applications

Concentration and Desalting of Chromatography Column Eluates, Concentrating Monoclonal Antibodies, Concentrating Proteins or Viruses From Culture Supernatants, Clarifying Tissue Homogenates and Lysates, Buffer Exchange or Diafiltration