Millipore Sigma Vibrant Logo

219474 Cathepsin G Substrate IV, Fluorogenic - Calbiochem

An internally quenched fluorogenic peptide substrate (Mca/Anb as fluorophore/quencher) that is preferentially cleaved by cathepsin G between Gnf and Ser with high efficiency and selectivity.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Mca-FVT-Gnf-SW-Anb-NH₂

219474
가격 및 재고 조회

개요

Replacement Information

가격 및 재고여부

카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
219474-1MGCN
재고 정보 검색...
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      		 Plastic ampoule 1 mg
      가격 검색...
      가격을 검색할 수 없습니다
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      가격 문의 추가 정보
      ()이 할인됨
       
      —
      가격 문의
      		 —
      Description
      OverviewAn internally quenched fluorogenic peptide substrate (Mca/Anb as fluorophore/quencher) that is preferentially cleaved by cathepsin G between Gnf and Ser with high efficiency and selectivity (kcat = 0.524 s-1; KM = 2.08 µM; kcat/KM = 252,000 M-1s-1) as well as sensitivity (≤70 pM Cath G; [subsrate] = 3.3 µM). Human neutrophil elastase and proteinase 3 are found to cleave the peptide between Val and Thr with a much slower kinetics (no detectable cleavage in 1 h) and lower efficiency (10% and 23% cleavage, respectively, after 24 h). Excitation max: 325 nm; emission max: 400 nm.
      Mca = 7-Methoxycoumarin-4-yl acetyl; Gnf = 4-Guanidine-L-phenylalanine; Anb = Amino nitrobenzoic acid.
      Catalogue Number219474
      Brand Family Calbiochem®
      SynonymsMca-FVT-Gnf-SW-Anb-NH₂
      References
      ReferencesLesner, A., et al. 2008. Anal. Biochem. 375, 306.
      Product Information
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt
      Hill FormulaC₆₁H₆₇N₁₃O₁₅
      Chemical formulaC₆₁H₆₇N₁₃O₁₅
      Hygroscopic Hygroscopic
      Quality LevelMQ100
      Applications
      Biological Information
      Purity≥97% by HPLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Peptide SequenceMca-Phe-Val-Thr-Gnf~Ser-Trp-Anb-NH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Shipped with Blue Ice or with Dry Ice
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Hygroscopic Hygroscopic
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      219474-1MGCN 04055977218510

      Documentation

      Cathepsin G Substrate IV, Fluorogenic - Calbiochem Certificates of Analysis

      TitleLot Number
      219474

      References

      참고문헌 보기
      Lesner, A., et al. 2008. Anal. Biochem. 375, 306.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision28-July-2020 JSW
      SynonymsMca-FVT-Gnf-SW-Anb-NH₂
      DescriptionAn internally quenched fluorogenic peptide substrate (Mca/Anb as fluorophore/quencher) that is preferentially cleaved by cathepsin G between Gnf and Ser with high efficiency and selectivity (kcat = 0.524 s-1; KM = 2.08 µM; kcat/KM = 252,000 M-1s-1) as well as sensitivity (≤70 pM Cath G; [subsrate] = 3.3 µM). Human neutrophil elastase and proteinase 3 are found to cleave the peptide between Val and Thr with a much slower kinetics (no detectable cleavage in 1 h) and lower efficiency (10% and 23% cleavage, respectively, after 24 h). Excitation max: 325 nm; Emission max: 400 nm
      FormLyophilized solid
      FormulationSupplied as a trifluoroacetate salt
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₆₁H₆₇N₁₃O₁₅
      Peptide SequenceMca-Phe-Val-Thr-Gnf~Ser-Trp-Anb-NH₂
      Purity≥97% by HPLC
      SolubilityDMSO (10 mg/ml)
      Storage Protect from light
      -20°C
      Hygroscopic
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C.
      Toxicity Standard Handling
      ReferencesLesner, A., et al. 2008. Anal. Biochem. 375, 306.