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QIA58 BrdU Cell Proliferation Assay

This proliferation assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells.

BrdU Cell Proliferation Assay MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Bromodeoxyuridine Assay

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QIA58
가격 및 재고 조회

개요

주요 사양표

Detection Methods
Colorimetric

가격 및 재고여부

카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
QIA58-1000TESTCN
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제한된 수량 가능
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      Contact Customer Service

      		 Fibre case 1000 test
      가격을 검색할 수 없습니다
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      가격 문의 추가 정보
      ()이 할인됨
       
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      즐겨찾기에 추가
      QIA58-200TESTCN
      —
      현재 재고 없음 현재 재고 없음
      예상 출고 가능일 
      단종품
      제한된 수량 가능
      재고여부 확인
        Remaining: will advise
          Remaining: will advise
          추천사항
          고객 서비스로 문의
          Contact Customer Service

          		 Fibre case 200 test
          가격을 검색할 수 없습니다
          Minimum Quantity needs to be mulitiple of
          Maximum Quantity is
          가격 문의 추가 정보
          ()이 할인됨
           
          —
          가격 문의
          		 —
          즐겨찾기에 추가
          Description
          OverviewBrdU Cell Proliferation Assay is a non-isotopic immunoassay for quantification of BrdU incorporation into newly synthesized DNA of actively proliferating cells. It is sensitive, rapid, and easy to perform.
          Note: 1 T = 1 test.
          Catalogue NumberQIA58
          Brand Family Calbiochem®
          SynonymsBromodeoxyuridine Assay
          Materials Required but Not Delivered• 2-20 µl, 20-200 µl and 200-1000 µl precision pipetters with disposable tips
          • Wash bottle or multichannel dispenser for washing
          • 500 ml graduated cylinder
          • PBS (137 mM NaCl, 2.7 mM KCl, 4.3 mM Na2HPO4-7H2O, 1.4 mM KH2PO4)
          • Deionized or distilled H2O
          • Spectrophotometer capable of measuring absorbance in 96-well plates using dual wavelength of 450-540 or 450-595 nm
          • Tissue culture plate (96 well culture dish)
          • Reagent trough
          • Micro filter (0.2 µm)
          • Syringe
          References
          ReferencesHardonk, M.J. and Harms, G. 1990. Acta histochemica, Suppl. 39, 99.
          Muir, D., et al. 1990. Analytical Biochemistry 185, 377.
          Lanier, T. L., et al. 1989. Carcinogenesis 10, 1341.
          Magaud, J.-P., et al. 1988. J. Immunol. Methods 106, 95.
          Collins, S.J. 1987. Blood 70, 1233.
          Porstmann, T., et al. 1985. J. Immunol. Methods 82, 169.
          Raza, A., et al. 1985. Cytometry 6, 633.
          Morstyn, G., et al. 1983. J. Clin. Invest. 72, 1844.
          Gratzner, H.G. 1982. Science 218, 474.
          Product Information
          Detection methodColorimetric
          Form200 or 1000 Tests
          Format96-well plate
          Kit containsBrdU Label, Fixative/Denaturing Solution, BrdU Antibody, Antibody Diluent, Peroxidase Goat Anti-Mouse IgG, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, and a user protocol.
          Quality LevelMQ100
          Biological Information
          Assay time3 h
          Sample TypeIntact Cells
          Safety Information
          R PhraseR: 11-20/21/22-35-36/38-46-61

          Highly flammable.
          Harmful by inhalation, in contact with skin and if swallowed.
          Causes severe burns.
          Irritating to eyes and skin.
          May cause heritable genetic damage.
          May cause harm to the unborn child.
          S PhraseS: 7-16-26-36/37/39-45

          Keep container tightly closed.
          Keep away from sources of ignition - No Smoking.
          In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
          Wear suitable protective clothing, gloves and eye/face protection.
          In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible).
          Product Usage Statements
          Intended useThe Calbiochem® BrdU Cell Proliferation Assay is a non-isotopic immunoassay for the quantitation of bromodeoxyuridine incorporation into newly synthesized DNA of actively proliferating cells.
          Storage and Shipping Information
          Ship Code Dry Ice Only
          Toxicity Multiple Toxicity Values, refer to MSDS
          Hazardous Materials Attention: Due to the nature of the Hazardous Materials in this shipment, additional shipping charges may be applied to your order. Certain sizes may be exempt from the additional hazardous materials shipping charges. Please contact your local sales office for more information regarding these charges.
          Storage -20°C
          Storage ConditionsUpon arrival store the entire contents of the kit at -20°C, in a non-frostfree freezer.

          Prior to use:
          1. Remove the Fixative/Denaturing Solution and place at room temperature for at least 4 h prior to use. Precipitates that may occur while cold should go back into solution.
          2. Reconstitute the Peroxidase Goat Anti-Mouse IgG with the appropriate volume of 1X PBS.
          • For the 200 test kit, reconstitute Peroxidase Goat Anti-Mouse IgG in 125 µl of 1X PBS. Use Conjugate Diluent for any further lot-specific dilution.
          • For the 1000 test kit, reconstitute Peroxidase Goat Anti-Mouse IgG in 250 µl of 1X PBS. Use Conjugate Diluent for any further lot-specific dilution.
          Allow solution to stand at room temperature for 10 min. (or until solution is clear). Divide into small aliquots; aliquots not to be used that same day should be stored at -20°C in a non-frostfree freezer.

          Once the kit components have been thawed for use:
          • The BrdU label and 100X Anti-BrdU Antibody should be divided into small aliquots and stored at -20°C in a non-frostfree freezer with the Peroxidase Goat Anti-Mouse IgG; avoid multiple freeze/thaw cycles.
          • All the other kit components (Substrate, Antibody Diluent, Conjugate Diluent, 20X Plate Wash Concentrate, Fixative/Denaturing Solution, Stop Solution) should be stored at 4°C.
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Supplemental Information
          Kit containsBrdU Label, Fixative/Denaturing Solution, BrdU Antibody, Antibody Diluent, Peroxidase Goat Anti-Mouse IgG, Conjugate Diluent, Substrate, Plate Wash Concentrate, Stop Solution, and a user protocol.
          Global Trade Item Number
          카탈로그 번호 GTIN
          QIA58-1000TESTCN 04055977209129
          QIA58-200TESTCN 04055977209136