수량	카탈로그 번호	주문 설명	포장 단위	기재 가격
			96-Well Plate

수량	카탈로그 번호	주문 설명	포장 단위	기재 가격
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP01A Sigma-AldrichAnti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate

This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate is validated for use in Immunoprecipitation, Other Applications for the detection of v-H-Ras (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet
Citations

Recommended Products

개요

Replacement Information

주요 사양표

Species Reactivity	Host	Antibody Type
H, M, R	R	Monoclonal Antibody

가격 및 재고여부

카탈로그 번호	재고 정보	패킹	포장 단위	가격(VAT 별도)	수량
OP01A-0.5MLCN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Plastic ampoule	.5 ml	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가

Description
Overview	Recognizes the ~21 kDa ras protein in SW480 and Y1 cells and normal skin tissue. For neutralization studies, Cat. No. OP01L is recommended. For immunoprecipitation, Cat. No. OP01A is recommended.
Catalogue Number	OP01A
Brand Family	Calbiochem®

References
References	Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

Product Information
Form	Liquid suspension
Formulation	In PBS.
Positive control	SW 480 or Y1 cells or normal skin tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.
Key Applications	Immunoprecipitation
Application Notes	Immunoprecipitation (15 µl beads or 1 µg antibody per sample, see comments) Other Applications (see comments)
Application Comments	Agarose conjugated recommended for immunoprecipitation. For frozen sections, paraffin sections, immunoblotting, neutralization, and immunofluoresence use Cat. No. OP01 or OP01L. Broadly reactive by immunoprecipitation with both phosphorylated and non-phosphorylated forms of v-H-ras and v-K-ras (see application references) and reacts with the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl agarose conjugate is recommended to be used as an immunoconcentrator prior to western blot analysis of p21^ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant p21 Ras
Clone	Y13-259
Host	Rat
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
카탈로그 번호	GTIN
OP01A-0.5MLCN	04055977209556

Documentation

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate MSDS

타이틀
물질안전보건자료(MSDS)

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Agarose Conjugate Certificates of Analysis

Title	Lot Number
OP01A	로트(lot) 번호를 입력하십시오.

References

참고문헌 보기
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

Citations

타이틀

Clair C. Sharpe, et al. (1999) Evidence of a Role for Ki-RAS in the Stimulated Proliferation of Renal Fibroblasts. Journal of the American Society of Nephrology 10, 1186-1192.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-October-2007 RFH
Application	Immunoprecipitation (15 µl beads or 1 µg antibody per sample, see comments) Other Applications (see comments)
Description	Rat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Conjugated to agarose. Recognizes the ~21 kDa ras protein (see comments).
Background	Oncogene sequences originally isolated from Harvey sarcoma virus v-H-ras and Kirsten sarcoma virus v-K-ras, both encode 21,000 dalton phosphoproteins (p21's) with guanine nucleotide binding activity. Human cellular homologues of v-H-ras and v-K-ras, designated c-H-ras and c-K-ras, respectively, and a related human oncogene, c-N-ras, each encode 21,000 dalton protein analogues to the gene products of their viral counterparts.
Host	Rat
Immunogen	recombinant p21 Ras
Clone	Y13-259
Isotype	IgG₁
Species	human, mouse, rat
Positive control	SW 480 or Y1 cells or normal skin tissue
Form	Liquid suspension
Formulation	In PBS.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Agarose conjugated recommended for immunoprecipitation. For frozen sections, paraffin sections, immunoblotting, neutralization, and immunofluoresence use Cat. No. OP01 or OP01L. Broadly reactive by immunoprecipitation with both phosphorylated and non-phosphorylated forms of v-H-ras and v-K-ras (see application references) and reacts with the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl agarose conjugate is recommended to be used as an immunoconcentrator prior to western blot analysis of p21^ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.
Citation	Clair C. Sharpe, et al. (1999) Evidence of a Role for Ki-RAS in the Stimulated Proliferation of Renal Fibroblasts. Journal of the American Society of Nephrology 10, 1186-1192.
Application references	Immunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.

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설명

OP01

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259)

가격 및 재고 조회

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