수량	카탈로그 번호	주문 설명	포장 단위	기재 가격
			96-Well Plate

수량	카탈로그 번호	주문 설명	포장 단위	기재 가격
	48-602MAG	Buffer Detection Kit for Magnetic Beads	1 Kit

OP01 Sigma-AldrichAnti-v-H-Ras (Ab-1) Rat mAb (Y13-259)

This Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) is validated for use in Frozen Sections, Immunoblotting, IF, IP, Paraffin Sections, Neutralization Studies for the detection of v-H-Ras (Ab-1).

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

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개요

Replacement Information

주요 사양표

Species Reactivity	Host	Antibody Type
H, M, R	R	Monoclonal Antibody

가격 및 재고여부

카탈로그 번호	재고 정보	패킹	포장 단위	가격(VAT 별도)	수량
OP01-100UGCN	재고 정보 검색... — 현재 재고 없음 예상 출고 가능일 단종품 제한된 수량 가능 재고여부 확인 재고목록을 보려면 로그인하십시오 예상 출고 가능일 현재 재고 없음 예상 출고 가능일 Remaining: will advise Remaining: will advise 추천사항 고객 서비스로 문의 Contact Customer Service 고객 서비스로 문의 고객 서비스로 문의	Plastic ampoule	100 μg	가격 검색... 가격을 검색할 수 없습니다 Minimum Quantity needs to be mulitiple of Maximum Quantity is 가격 문의 추가 정보 ()이 할인됨 — 가격 문의 고객님의 가격을 보려면 로그인하십시오	재고 조회 —	즐겨찾기에 추가 즐겨찾기에 추가 나의 즐겨찾기에 추가

Description
Overview	Recognizes both phosphorylated and nonphosphorylated forms of v-H-Ras, and v-K-Ras in SW480 and Y1 cells and normal skin tissue. Also recognizes H, K, and N-Ras human translated products.
Catalogue Number	OP01
Brand Family	Calbiochem®

References
References	Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

Product Information
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Positive control	SW480 or Y1 cells or normal skin tissue
Preservative	≤0.1% sodium azide
Quality Level	MQ100

Applications
Application References	Immunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.
Key Applications	Frozen Sections Immunoblotting (Western Blotting) Immunofluorescence Immunoprecipitation Neutralization Studies Paraffin Sections
Application Notes	Frozen Sections (5 µg/ml) Immunoblotting (2.5 µg/ml, colormetric or chemiluminescence) Immunofluorescence (2.5 µg/ml) Immunoprecipitation (use Cat. No. OP01A or 1 µg antibody per sample) Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required) Neutralization Studies (see application references)
Application Comments	Broadly reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras (see application references) and recognizes the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl v-H-ras (Ab-1) agarose conjugate (Cat. No. OP01A) is recommended to be used as an immunoconcentrator prior to immunoblot analysis of p21^ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.

Biological Information
Immunogen	recombinant p21 Ras
Epitope	within amino acids 62-76
Clone	Y13-259
Host	Rat
Isotype	IgG₁
Species Reactivity	Human Mouse Rat
Antibody Type	Monoclonal Antibody
Concentration Label	Please refer to vial label for lot-specific concentration

Physicochemical Information

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information

Product Usage Statements

Storage and Shipping Information
Ship Code	Blue Ice Only
Toxicity	Standard Handling
Storage	+2°C to +8°C
Do not freeze	Yes

Packaging Information

Transport Information

Supplemental Information

Specifications

Global Trade Item Number
카탈로그 번호	GTIN
OP01-100UGCN	04055977209570

Documentation

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) MSDS

타이틀
물질안전보건자료(MSDS)

Anti-v-H-Ras (Ab-1) Rat mAb (Y13-259) Certificates of Analysis

Title	Lot Number
OP01	로트(lot) 번호를 입력하십시오.

References

참고문헌 보기
Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	01-October-2007 RFH
Application	Frozen Sections (5 µg/ml) Immunoblotting (2.5 µg/ml, colormetric or chemiluminescence) Immunofluorescence (2.5 µg/ml) Immunoprecipitation (use Cat. No. OP01A or 1 µg antibody per sample) Paraffin Sections (5 µg/ml, saponin or pepsin pre-treatment required) Neutralization Studies (see application references)
Description	Purified rat monoclonal antibody generated by immunizing Sprague Dawlay rats with the specified immunogen and fusing splenocytes with Y3Ag1.2.3 rat myeloma cells. Recognizes the ~21 kDa Ras protein (see comments).
Background	Oncogene sequences originally isolated from Harvey sarcoma virus v-H-ras and Kirsten sarcoma virus v-K-ras, both encode 21,000 dalton phosphoproteins (p21's) with guanine nucleotide binding activity. Human cellular homologues of v-H-ras and v-K-ras, designated c-H-ras and c-K-ras, respectively, and a related human oncogene, c-N-ras, each encode 21,000 dalton protein analogues to the gene products of their viral counterparts.
Host	Rat
Immunogen	recombinant p21 Ras
Epitope	within amino acids 62-76
Clone	Y13-259
Isotype	IgG₁
Species	human, mouse, rat
Positive control	SW480 or Y1 cells or normal skin tissue
Form	Liquid
Formulation	In 0.05 M sodium phosphate buffer, 0.2% gelatin.
Concentration Label	Please refer to vial label for lot-specific concentration
Preservative	≤0.1% sodium azide
Comments	Broadly reactive with the phosphorylated and non-phosphorylated forms of v-H-Ras and v-K-Ras (see application references) and recognizes the p21 translational products of the H-, K-, and N-ras human genes (see application references). This antibody does not cross-react with rap. Neutralizes the biological and biochemical activities of H-, K-, and N-ras p21 of vertebrates by binding to residues Glu-63, Ser-65, Ala-66, Met-67, Gln-70, and Arg-73 (see application references). 15 µl v-H-ras (Ab-1) agarose conjugate (Cat. No. OP01A) is recommended to be used as an immunoconcentrator prior to immunoblot analysis of p21^ras with another antibody. To immunoprecipitate with the unconjugated antibody, use Protein A with a rabbit anti-rat IgG or Protein G PLUS with a goat anti-rat IgG. Protein A alone will not immunoprecipitate rat IgG. A doublet may be detected corresponding to ras protein with and without farnesyl group. Antibody should be titrated for optimal results in individual systems.
Storage	+2°C to +8°C
Do Not Freeze	Yes
Toxicity	Standard Handling
References	Shimizu, K., et al. 1983. Proc. Natl. Acad. Sci. USA 80, 2112. Taparowsky, E., et al. 1983. Cell 34, 581. Ellis, R.W., et al. 1981. Nature 292, 506. Shih, T.Y., et al. 1980. Nature 287, 686.
Application references	Immunoprecipitation Furth, M.E., et al. 1982. J.Virol. 43, 294. Paraffin Sections, Immunoprecipitation, and Immunoblotting Furth, M.E., et al. 1987. Oncogene 1 47. Neutralization Signal, I.S., et al. 1986. Proc. Natl. Acad. Sci. USA 83, 4725. Specificity Furth, M.E., et al. 1987. Oncogene 1, 47. Furth, M.E., et al. 1982. J. Virol. 43, 294.

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