Anti-phospho NFkB p65 Antibody, (Ser547)

NF-kappa-B is a pleiotropic transcription factor present in almost all cell types and is the endpoint of a series of signal transduction events that are initiated by a vast array of stimuli related to many biological processes such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa B forms multiple homo and heterodimeric complexes, one of which is with RELA/p65. The NF-kappa-B p65 complex appears to be involved in invasin-mediated activation of IL-8 expression, as well as the inhibitor effect on I-kappa-B. Phosphorylation of p65 at serine 547 by the nuclear kinase ATM appears to repress NF-κB-dependent transcription of specific genes induced by genotoxic stress.

~62 kDa observed. Uncharacterized band(s) may appear in some lysates.

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human phospho NFkB p65, (Ser547).

Anti-phospho NFkB p65 Antibody, (Ser547) is an antibody against phospho NFkB p65 Antibody for use in Western Blotting.

Research Category
Signaling

Research Sub Category
Signaling Neuroscience

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Western Blotting in p65 WT transfected U2OS cell lysate.

Western Blotting Analysis: A 1:5,000 dilution of this antibody detected phospho NFkB p65, (Ser547) in 10 µg of p65 WT transfected U2OS cell lysate.

Concentration: Please refer to lot specific datasheet.

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