Anti-p53 (pantropic) Antibody, clone DO-1

Cellular tumor antigen p53 (p53) is a ubiquitous transcription factor that is most widely known for its function as a tumor suppressor, through its regulation of cell growth and survival. p53 is activated in response to cell stress such as DNA damage and induces either cell cycle arrest or apoptosis. It coordinates the expression of multiple genes including p21, GADD45, Bax, and Puma. p53 is regulated by multiple mechanisms: At the post-transcriptional level, the stability of p53 mRNAs is regulated by the binding of molecules such as HuR, Wig-1, RPL26, L26, and WRAP53. Post-translationally, p53 undergoes phosphorylation and acetylation which may also play a role in regulating its activity. It may also undergo ubiquitination via the Akt-MDM2 pathway resulting in degradation by the 20S proteosome. Mutations in p53 are prevalent in many types of cancers, underscoring the therapeutic relevance of this protein.

~53 kDa observed. Uniprot describes 9 different isoforms due to alternative splicing and post-translational modification. This antibody detects isoform 1 (~38 kDa), isoform 2 (~39 kDa), and isoform 3 (~39 kDa). An uncharacterized band may be observed at ~43 kDa in some cell lysates.

Epitope: N-terminus

Recombinant protein corresponding to human p53 (pantropic).

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected p53 (pantropic) in A431 cells.

Immunohistochemistry Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal adenocarcinoma tissue and in human prostate cancer tissue.

Immunofluorescence Analysis: A 1:1,000 dilution from a representative lot detected p53 (pantropic) in human colorectal cancer cells.

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in radiolabelled HOS cell lysate (Vojtesek, B., et al. (1995). 71(6):1253-1256.).

Immunohistochemistry Analysis: A representative lot was used by an independent laboratory in primary breast carcinoma tissue (Beck, T., et al. (1995). Gynecol Oncol. 57(1):96-104.).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Cell Cycle, DNA Replication & Repair

Use Anti-p53 (pantropic) Antibody, clone DO-1 (Mouse Monoclonal Antibody) validated in WB, ICC, IHC, IF, IP to detect p53 (pantropic) also known as Cellular tumor antigen p53 or Antigen NY-CO-13.

This antibody recognizes the N-terminus of p53 (pantropic).

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG2aκ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
A431 cell lysate

Evaluated by Western Blot in A431 cell lysate.

Western Blot Analysis: 1 µg/mL of this antibody detected p53 (pantropic) in 10 µg of A431 cell lysate. This antibody detects isoforms 1, 2, and 3.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: 04-1083

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.