Millipore Sigma Vibrant Logo

OP03 Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421)

This Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) is validated for use in Frozen Sections, Gel Shift, Immunoblotting, IF, FC, IP for the detection of p53 (Ab-1) (Pantropic).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
OP03
가격 및 재고 조회

개요

Replacement Information

주요 사양표

Species ReactivityHostAntibody Type
H, Mk, M, Rb, RMMonoclonal Antibody

가격 및 재고여부

카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
OP03-100UGCN
재고 정보 검색...
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      		 Glass bottle 100 μg
      가격 검색...
      가격을 검색할 수 없습니다
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      가격 문의 추가 정보
      ()이 할인됨
       
      —
      가격 문의
      		 —
      OP03-20UGCN
      재고 정보 검색...
      —
      현재 재고 없음 현재 재고 없음
      예상 출고 가능일 
      단종품
      제한된 수량 가능
      재고여부 확인
        Remaining: will advise
          Remaining: will advise
          추천사항
          고객 서비스로 문의
          Contact Customer Service

          		 Glass bottle 20 μg
          가격 검색...
          가격을 검색할 수 없습니다
          Minimum Quantity needs to be mulitiple of
          Maximum Quantity is
          가격 문의 추가 정보
          ()이 할인됨
           
          —
          가격 문의
          		 —
          Description
          OverviewRecognizes the ~53 kDa mammalian wild-type and mutant p53 protein. Does not recognize phosphorylated or acetylated p53.
          Catalogue NumberOP03
          Brand Family Calbiochem®
          References
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
          Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
          Harlow, E., et al. 1981. J. Virol. 39 861.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative controlSK-OV-3 cells or normal skin
          Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. (within amino acids 371-380) Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.
          Key Applications Flow Cytometry
          Frozen Sections
          Gel Shift
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Application NotesFrozen Sections (10 µg/ml, see application references)
          Gel Shift (see comments)
          Immunoblotting (10 µg/ml, see application references)
          Immunofluorescence (1-20 µg/ml, see application references)
          Flow Cytometry (1-20 µg/ml)
          Immunoprecipitation (1 μg per sample, see application references)
          Application CommentsOriginally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenpartially purified mouse p53
          ImmunogenMouse
          Epitopewithin amino acids 376-378 of human p53
          ClonePAb421
          HostMouse
          IsotypeIgG2a
          Species Reactivity
          • Human
          • Monkey
          • Mouse
          • Rabbit
          • Rat
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          카탈로그 번호 GTIN
          OP03-100UGCN 04055977209624
          OP03-20UGCN 04055977209631

          Documentation

          Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) MSDS

          타이틀

          물질안전보건자료(MSDS) 

          Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) Certificates of Analysis

          TitleLot Number
          OP03

          References

          참고문헌 보기
          El-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
          Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
          Harlow, E., et al. 1981. J. Virol. 39 861.

          Brochure

          Title
          Caspases and other Apoptosis Related Tools Brochure
          Interactive Pathway Calendar
          Data Sheet

          Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

          Revision17-July-2008 JSW
          ApplicationFrozen Sections (10 µg/ml, see application references)
          Gel Shift (see comments)
          Immunoblotting (10 µg/ml, see application references)
          Immunofluorescence (1-20 µg/ml, see application references)
          Flow Cytometry (1-20 µg/ml)
          Immunoprecipitation (1 μg per sample, see application references)
          DescriptionPurified mouse monoclonal antibody generated by immunizing mice with the specified immunogen and fusing splenocytes with NS1 mouse myeloma cells (see application references). Recognizes the ~53 kDa mutant and wild-type p53 proteins.
          BackgroundThe human p53 tumor suppressor gene encodes a 393 amino acid phosphoprotein that exhibits sequence-specific DNA binding and directly interacts with various cellular and viral proteins. p53 is the most commonly mutated gene in human cancer, with the majority of the mutations being amino acid substitutions. The normal function of p53 is to effect cell cycle arrest at the G1 and G2 checkpoints in response to DNA damage. This checkpoint function is executed by accumulation of p53 followed by induction of the GADD45, WAF1 and MDM2 genes. The current model of p53 function postulates that p53 senses DNA damage and arrests the cell cycle in either the G1 or G2 phases to allow DNA repair to take place. If repair is not successful, p53 initiates programmed cell death, thus preventing the propagation of genetic defects to successive generations of cells.
          HostMouse
          Immunogen speciesMouse
          Immunogenpartially purified mouse p53
          Epitopewithin amino acids 376-378 of human p53
          ClonePAb421
          IsotypeIgG2a
          Specieshuman, monkey, mouse, rabbit, rat
          Positive controlBreast carcinoma, A431 cells, or p53 standard (Cat. No. 506147)
          Negative controlSK-OV-3 cells or normal skin
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Concentration Label Please refer to vial label for lot-specific concentration
          Preservative≤0.1% sodium azide
          CommentsOriginally called clone L21. Does not recognize phosphorylated p53. For a gel shift assay, use Cat. No. OP03L and resuspend in 100 µl buffer. Wild-type p53 has a short half-life and is present in low amounts in cells. For immunoprecipitation, increasing the amount of sample to be immunoprecipitated and applied to the gel may help visualize wild-type p53, and short incubation times with 35S-Met (≤1 h) will help reduce background. For immunoblotting of wild-type p53, maximize sensitivity by concentrating samples by immunoprecipitation with Cat. No. OP03, then immunoblot using PC35 and chemiluminescent detection. This antibody recognizes the region encompassing Ser376 to Ser378 (see application references) and does not recognize phosphorylated p53 (see application references). Antibody should be titrated for optimal results in individual systems.
          Storage +2°C to +8°C
          Do Not Freeze Yes
          Toxicity Standard Handling
          ReferencesEl-Deiry, W.S., et al. 1994. Cancer Res. 54, 1169.
          Greenblatt, M.S., et al. 1994. Cancer Res. 54, 4855.
          Legros, Y., et al. 1994. Oncogene 9, 2071.
          Barak, Y., et al. 1993. EMBO J. 12, 461.
          Kuerbitz, S.J. 1992. Proc. Natl. Acad. Sci. USA 89, 7491.
          Kastan, M.B., et al. 1992. Cell 71, 587.
          Lane, D.P. 1992. Nature 358, 15.
          Kastan, M.B., et al. 1991. Cancer Res. 51 6304.
          Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709.
          Harlow, E., et al. 1981. J. Virol. 39 861.
          Application referencesImmunoblotting Wang, H., et al. 1991. Cell Growth and Diff. 2, 645. Immunofluorescence, Frozen Sections Davidoff, A.M., et al. 1991. Proc. Natl. Acad. Sci. USA 88, 5006. Immunoprecipitation Lehman, T.A., et al. 1991. Cancer Res. 51, 4090. Harlow, E., et al. 1981. J. Virol. 39, 861. Original Clone Crawford, L. and Harlow, E. 1982. J. Virol. 41, 709. Harlow, E., et al. 1981. J. Virol. 39 861. Epitope Identification Legros, Y., et al. 1994. Oncogene 9, 2071. (within amino acids 371-380) Waterman, M.J.F., et al. 1998. Nature Genetics 19, 175. (within amino acids 376-378) Cross Reactivity/Phosphophorylated p53/Acetylated p53 Li, M., et al. 2002. J. Biol. Chem. 227, 50607. Delphin, C. and Baudier, J. 1994. J. Biol. Chem. 269, 29579.

          관련 제품 및 어플리케이션

          Alternative Products

          카탈로그 번호 설명  
          OP09 Anti-p53 (Ab-2) (Pantropic) Mouse mAb (PAb1801) 가격 및 재고 조회
          OP33 Anti-p53 (Ab-5) (Wild type) Mouse mAb (PAb1620) 가격 및 재고 조회

          Related Products

          카탈로그 번호 설명  
          OP03L Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) 가격 및 재고 조회

          Related Products

          카탈로그 번호 설명  
          OP03L Anti-p53 (Ab-1) (Pantropic) Mouse mAb (PAb421) 가격 및 재고 조회

          카테고리

          Life Science Research > Antibodies and Assays > Primary Antibodies