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OP64 Anti-p21WAF1 (Ab-1) Mouse mAb (EA10)

This Anti-p21WAF1 (Ab-1) Mouse mAb (EA10) is validated for use in FC, Immunoblotting, IF, IP, Paraffin Sections for the detection of p21WAF1 (Ab-1).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-CIP1, Anti-SD11, Anti-p21, Anti-WAF

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OP64
가격 및 재고 조회

개요

주요 사양표

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

가격 및 재고여부

카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
OP64-100UGCN
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      		 Plastic ampoule 100 μg
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      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
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      OP64-20UGCN
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      현재 재고 없음 현재 재고 없음
      예상 출고 가능일 
      단종품
      제한된 수량 가능
      재고여부 확인
        Remaining: will advise
          Remaining: will advise
          추천사항
          고객 서비스로 문의
          Contact Customer Service

          		 Plastic ampoule 20 μg
          가격을 검색할 수 없습니다
          Minimum Quantity needs to be mulitiple of
          Maximum Quantity is
          가격 문의 추가 정보
          ()이 할인됨
           
          —
          가격 문의
          		 —
          즐겨찾기에 추가
          Description
          OverviewRecognizes the ~21 kDa p21WAF1 protein in skin and colon tissue and in cells expressing wild-type p53 (e.g. Hs27 or U205 cells treated with DNA damaging agents).
          Catalogue NumberOP64
          Brand Family Calbiochem®
          SynonymsAnti-CIP1, Anti-SD11, Anti-p21, Anti-WAF
          Application Data
          Detection of p21 WAF1 by immunoblotting: Sample: Whole cell lysate (56 µg) from MCF-7 cells. Primary antibody: Anti-p21WAF1 (Ab-1) Mouse mAb (EA10) (Cat. No. OP64) (3 µg/ml). Secondary antibody: Anti-Mouse IgG (Goat) Peroxidase Conjugate. Detection: chemiluminescence.
          References
          ReferencesAgarwal, M.L., et al. 1995. Proc. Natl. Acad. Sci. USA 92, 8493.
          Chen, Y.Q., et al. 1995. Int. J. Oncology 7, 889.
          Deng, C., et al. 1995. Cell 82, 675.
          El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910.
          Waldman, T., et al. 1995. Cancer Res. 55, 5187.
          Elbendary, A., et al.1994. Cell Growth Diff. 5, 1301.
          El-Deiry, W.S., et al. 1994 Cancer Res. 54, 1169.
          Li, R., et al. 1994. Nature 371, 534.
          Michieli, P., et al. 1994. Cancer Res. 54, 3391.
          Noda, A., et al.1994. Exp. Cell Res. 211, 90.
          El-Deiry, W.S., et al.1993. Cell 75, 817.
          Gu, Y., et al. 1993. Nature 366, 707.
          Harper, J.W., et al.1993. Cell 75, 805.
          Xiong, Y., et al.1993. Genes Devel. 7, 1572.
          Xiong, Y., et al.1993. Nature 366, 701.
          Xiong, Y., et al.1992. Cell 71, 505.
          Product Information
          FormLiquid
          FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
          Negative controlUnstimulated cells, unstimulated skin tissue, or SK-OV-3 cells
          Positive controlAny cell line expressing wild-type p53 (e.g. Hs27 or U2OS treated with DNA-damaging agents) or skin or colon tissue
          Preservative≤0.1% sodium azide
          Quality LevelMQ100
          Applications
          Application ReferencesEpitope Identification Patrick O'Connor (NCI, personal communication). Original Clone, Paraffin Sections, Frozen Sections El-Deiry, W.S., et al. 1995. Cancer Res. 55, 2910. Paraffin Sections, Antigen Retrieval Mouriaux, Frédéric, et al. 2000. Invest Ophthamol. Vis. Sci. 41, 2837. Flow Cytometry Carter, A., et al. 2004. Brit. J. Haematol. 127, 425.
          Key Applications Flow Cytometry
          Frozen Sections
          Immunoblotting (Western Blotting)
          Immunofluorescence
          Immunoprecipitation
          Paraffin Sections
          Application NotesFlow Cytometry (2 µg/ml or use Cat. No. OP64F; see application references)
          Frozen Sections (5 µg/ml or use Cat. No. OP64F)
          Immunoblotting (1-3 µg/ml)
          Immunofluorescence (1-5 µg/ml or use Cat. No. OP64F)
          Immunoprecipitation (2 µg/sample)
          Paraffin Sections (5 µg/ml or use OP64F; heat pre-treatment required; see comments and application references)
          Application CommentsMaximal p21WAF1 expression requires wild type p53 activity. Treatment of U2OS or MCF7 cells with DNA damaging agents (such as doxorubicin at 0.2 µg/ml) induces wild type p53 expression which in turn activates WAF1 expression. Serum stimulation of quiescent cells will give low level WAF1 expression independent of p53 expression. Untreated cells will express little p21WAF1 and can be used as a negative control. Cat. No. OP64F was tested in HALT cells induced by incubation at 31°C; FITC-goat anti-mouse IgG (Cat. No. DC13L) was used as a negative control. This antibody will immunoprecipitate p21WAF1 but not associated proteins. For immunoblotting applications, use a 0.22 µm filter and visualize by chemiluminescence. For staining paraffin sections, heating the tissue in 10 mM citrate buffer is required (see application references). In either paraffin or frozen sections of normal human colon, the non-dividing cells of colonic epithelium will stain positive for p21WAF1 while the proliferating compartment of crypts will not stain. Antibody should be titrated for optimal results in individual systems.
          Biological Information
          Immunogenfull-length, recombinant, human p21WAF1
          ImmunogenHuman
          Epitopewithin amino acids 58-77 of human p21WAF1
          CloneEA10
          HostMouse
          IsotypeIgG₁
          Species Reactivity
          • Human
          Antibody TypeMonoclonal Antibody
          Concentration Label Please refer to vial label for lot-specific concentration
          Storage and Shipping Information
          Ship Code Blue Ice Only
          Toxicity Standard Handling
          Storage +2°C to +8°C
          Do not freeze Yes
          Global Trade Item Number
          카탈로그 번호 GTIN
          OP64-100UGCN 07790788053918
          OP64-20UGCN 04055977227291