Millipore Sigma Vibrant Logo

MABE351 Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5

Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 is a mouse monoclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as Histone H3.1t, H3/t, H3t, H3/g & has been validated in WB, IP, ICC.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Research Sub-Category: Histones Gene Symbol: HIST3H3, H3FT UniProt Number: Q16695
View Products on Sigmaaldrich.com
MABE351
100 µL  
가격 검색...
가격을 검색할 수 없습니다
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
가격 문의 추가 정보
()이 할인됨
 
—
가격 문의
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      특별 제공

       

      고객 서비스로 문의

      개요

      Replacement Information

      특별 제공

      100% Performance Guaranteed

      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWB, IP, ICC, ChIP-seq, ChIPMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABE351
      DescriptionAnti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5
      Alternate Names
      • Histone H3.1t
      • H3/t
      • H3t
      • H3/g
      Background InformationHistones are nuclear proteins that form octameric structures which bind DNA to form units of chromatin called nucleosomes. The family of histones—H2A, H2B, H3, and H4—are key players in gene regulation. They undergo a number of post-translational modifications (PTM) in response to various stimuli. Acetylation is a reversible PTM that involves the transfer of acetyl groups to N-terminal lysine (K) residues by a family of histone acetyltransferase enzymes, including GNAT, Gcn5, and p300/CBP. Histone acetylation reduces the electrostatic attractions between DNA and histones, which destabilizes chromatin structure and provide chromatin remodeling and transcriptional complexes, with access to DNA, potentially resulting in increased gene transcription. In fission yeast, acetylation of H3K4 is regulated by Gcn5 and the Mst2 complex, and is crucial to the activation of DNA damage checkpoint.
      References
      Product Information
      FormatAscites
      Control
      • HeLa acid extract
      PresentationMouse monoclonal IgG1 in ascites containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 is a mouse monoclonal antibody for detection of acetyl-Histone H3 (Lys14) also known as Histone H3.1t, H3/t, H3t, H3/g & has been validated in WB, IP, ICC.
      Key Applications
      • Western Blotting
      • Immunoprecipitation
      • Immunocytochemistry
      • ChIP-seq
      • Chromatin Immunoprecipitation (ChIP)
      Application NotesImmunoprecipitation Analysis: A representative lot was used to immunoprecipitate acetyl-Histone H3 (Lys4) in IP.

      Immunocytochemistry Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ICC.

      Chromatin Immunoprecipitation Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in ChIP.

      Western Blot Analysis: A representative lot was used to detect acetyl-Histone H3 (Lys4) in WB.
      Biological Information
      ImmunogenOvalbumin-conjugated linear peptide corresponding to human Histone H3 acetylated at Lys14.
      Cloneclone 13HH3-1A5
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteDemonstrated to react with Human.
      Demonstrated to react in Mouse ESCs in ChIP-seq in Karmodiya et al. BMC Genomics 2012, 13:424.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST3H3
      • H3FT
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~17 kDa observed. Uncharacterized bands may be observed at ~23 kDa and ~35 kDa in some cell lysates.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in HeLa acid extract.

      Western Blot Analysis: A 1:2,000 dilution of this antibody detected acetyl-Histone H3 (Lys4) in 10 µg of HeLa acid extract.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABE351 04053252697760

      Documentation

      Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-acetyl-Histone H3 (Lys14) Antibody, clone 13HH3-1A5 Certificates of Analysis

      TitleLot Number
      Anti-acetyl-Histone H3 (Lys14), -2520566 2520566
      Anti-acetyl-Histone H3 (Lys14), -2665808 2665808
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 3013345
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 2298448 2298448
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 3508428 3508428
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 3853802 3853802
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - 4204627 4204627
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 - Q2029943 Q2029943
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 -2705156 2705156
      Anti-acetyl-Histone H3 (Lys14), clone 13HH3-1A5 Monoclonal Antibody 2959567
      Show more

      References

      Reference overviewApplicationSpeciesPub Med ID
      H3K9 and H3K14 acetylation co-occur at many gene regulatory elements, while H3K14ac marks a subset of inactive inducible promoters in mouse embryonic stem cells.
      Karmodiya, Krishanpal, et al.
      BMC Genomics, 13: 424 (2012)  2012

      요약 표시       		Chromatin Immunoprecipitation (ChIP)Mouse22920947 22920947

      관련 제품 및 어플리케이션

      Related Products

      카탈로그 번호 설명  
      AP124P Goat Anti-Mouse IgG Antibody, Peroxidase Conjugated, H+L 가격 및 재고 조회

      카테고리

      Life Science Research > Antibodies and Assays > Primary Antibodies