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16-232 Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 488 conjugate

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16-232
100 µg  
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      개요

      Replacement Information

      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, R, Gp, B, M, Po, AvFC, ICC, IF, WBMAlexaFluor®488Monoclonal Antibody
      Description
      Catalogue Number16-232
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 488 conjugate
      Alternate Names
      • Alpha-tubulin 3
      • Tubulin B-alpha-1
      • Tubulin alpha-3 chain
      • tubulin, alpha 1a
      • tubulin, alpha 3
      • tubulin, alpha, brain-specific
      Background InformationTubulins, major components of the cellular cytoskeleton, form microtubules that are critical for cellular structure, migration, cell division and intracellular trafficking. There are at least three types of tubulins, alpha, beta and gamma. Each has a molecular weight of approximately 55 kDa. Microtubules are large structures (300 angstrom diameter) composed of alternating alpha and beta subunits. Compounds that block microtuble formation, such as Taxol and the vinca alkaloids, inhibit cell division and are effective anti-cancer agents.
      References
      Product Information
      FormatAlexaFluor®488
      Control
      • Included Negative Control: Catalog # 16-240, Normal Mouse IgG, Alexa Fluor® 488-conjugate.
      PresentationAlexa Fluor® 488 conjugated, purified mouse monoclonal IgG1 in buffer containing PBS containing 1% BSA, 0.05% Tween®-20, 0.05% sodium azide.
      Liquid at 2-8°C.
      Quality LevelMQ100
      Applications
      ApplicationAnti-α-Tubulin Antibody, clone DM1A, Alexa Fluor 488 conjugate is an antibody against α-Tubulin for use in FC, IC, IF & WB.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      • Western Blotting
      Application NotesWestern Blot Analysis:
      2 µg/mL of a previous lot detected α-Tubulin in RIPA lysates from A431 cells.

      Immunocytochemistry:
      2 µg/mL of a previous lot showed positive immunostaining for α-Tubulin in HeLa cells.
      Biological Information
      ImmunogenPurified human brain α-Tubulin. The epitope recognized by this antibody has been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
      EpitopeThe epitopehas been mapped to amino acids 426-450 (AALEKDYEEVGVDSV-EGEGEEEGEE).
      CloneDM1A
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      SpecificityRecognizes α-Tubulin, clone DM1A, Mr 50-60 kDa.
      IsotypeIgG1
      Species Reactivity
      • Human
      • Rat
      • Guinea Pig
      • Bovine
      • Mouse
      • Pig
      • Avian
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryMicrotubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The genes encoding these microtubule constituents belong to the tubulin superfamily, which is composed of six distinct families. Genes from the alpha, beta and gamma tubulin families are found in all eukaryotes. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. This gene encodes alpha tubulin and is highly similar to mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells. This gene is one of three alpha-tubulin genes in a cluster on chromosome 12q.
      Gene Symbol
      • TUBA1A
      • B-ALPHA-1
      • FLJ25113
      • hum-a-tub1
      • TUBA3
      • hum-a-tub2
      Purification MethodProtein G Purified
      UniProt Number
      UniProt SummaryFUNCTION:SwissProt: Q71U36 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
      SIZE:451 amino acids; 50136 Da
      SUBUNIT: Dimer of alpha and beta chains.
      PTM:Undergoes a tyrosination/detyrosination cycle, the cyclic removal and re-addition of a C-terminal tyrosine residue by the enzymes tubulin tyrosine carboxypeptidase (TTCP) and tubulin tyrosine ligase (TTL), respectively (By similarity).
      SIMILARITY:SwissProt: Q71U36 ## Belongs to the tubulin family.
      Molecular Weight50-60 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Flow Cytometry.

      Flow Cytometry:
      0.2 μg of this lot detected α-Tubulin in fixed and permeabilized rat L6, Jurkat, and A431 cells.
      Usage Statement
      • Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Do Not Freeze. Do not store the material diluted.
      For maximum recovery of product, centrifuge original vial prior to removing cap.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      16-232 04053252471315

      Documentation

      Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 488 conjugate MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 488 conjugate Certificates of Analysis

      TitleLot Number
      Anti--Tubulin, clone DM1A, Alexa Fluor#174; 488 conjugate (mouse monoclonal IgG1) - DAM1411293 DAM1411293
      Anti--Tubulin, clone DM1A, Alexa Fluor#174; 488 conjugate - 2065953 2065953
      Anti--Tubulin, clone DM1A, Alexa Fluor#174; 488 conjugate - 2106875 2106875
      Anti--Tubulin, clone DM1A, Alexa Fluor#174; 488 conjugate - 2147039 2147039
      Anti--Tubulin, clone DM1A, Alexa Fluor® 488 conjugate - 2273810 2273810
      Anti-alpha-Tubulin, clone DM1A, Alexa Fluor#174; 488 conjugate - DAM1479582 DAM1479582
      Anti-alpha;-Tubulin, clone DM1A, Alexa Fluorreg; 488 conjugate - 30486 30486
      Anti-alpha;-Tubulin, clone DM1A, Alexa Fluorreg; 488 conjugate - 33436 33436
      Anti-α-Tubulin, clone DM1A, 2397048
      Anti-α-Tubulin, clone DM1A, -2607282 2607282

      References

      Reference overviewPub Med ID
      cAMP, tubulin, axonal transport, and regeneration.
      Liu, Howard H and Brady, Scott T
      Exp. Neurol., 189: 199-203 (2004)  2004

      요약 표시
      15380472 15380472
      Crystallographic structure of tubulin: implications for dynamics and drug binding.
      Downing, K H and Nogales, E
      Cell Struct. Funct., 24: 269-75 (1999)  1999

      요약 표시
      15216882 15216882

      관련 제품 및 어플리케이션

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      Alternative Format

      카탈로그 번호 설명  
      05-829 Anti-α-Tubulin Antibody, clone DM1A 가격 및 재고 조회
      05-829X-555 Anti-α-Tubulin Antibody, Alexa Fluor® 555 conjugate 가격 및 재고 조회

      Included Negative Control

      카탈로그 번호 설명  
      16-240 Normal Mouse IgG, Alexa Fluor® 488 conjugate 가격 및 재고 조회

      카테고리

      Life Science Research > Antibodies and Assays > Primary Antibodies