Anti-Robo2 Antibody, clone 4C6.1

Roundabout homolog 2 (Robo2) is a receptor for SLIT2, and likely SLIT1, which are thought to help guide cellular migration, including axonal navigation of the neural tube and the projection of axons during neuronal development. Vesicoureteral reflux type 2 (VUR2) has been attributed to defects in Robo2.

~ 170 kDa observed. The calculated molecular weight is 150 kDa, however Robo2 has been shown as a ~170 kDa band in western blots due to high glycosylation. (Fricke, C., et al. (2001). Science. 292:(5516):507-510).

Epitope: Cytoplasmic domain

GST-tagged recombinant protein corresponding to the cytoplasmic domain of human Robo2.

Detect Roundabout homolog 2 using this mouse monoclonal antibody, Anti-Robo2 Antibody, clone 4C6.1 validated for use in western blotting & IHC (Paraffin).

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected Robo2 in rat brain (hypothalamus) and human brain tissue.

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

This antibody recognizes the cytoplasmic domain of Robo2

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Control
Mouse neuronal stem cell lysate

Evaluated by Western Blotting in mouse neuronal stem cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected Robo2 in 200 µg of mouse neuronal stem cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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