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Merck

MAB5326

Anti-Nestin Antibody, clone 10C2

clone 10C2, Chemicon®, from mouse

동의어(들):

Anti-Nestin

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크기 선택

제품정보 (DICE 배송 시 비용 별도)

UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702

주요 문서

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제품 이름

Anti-Nestin Antibody, clone 10C2, clone 10C2, Chemicon®, from mouse

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

clone

10C2, monoclonal

species reactivity

human

should not react with

rat, mouse

packaging

antibody small pack of 25 μg

manufacturer/tradename

Chemicon®

technique(s)

immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable

input

sample type epithelial cells
sample type neural stem cell(s)

isotype

IgG1

NCBI accession no.

NM_006617.1

UniProt accession no.

P48681

shipped in

ambient

storage temp.

2-8°C

target post-translational modification

unmodified

Quality Level

100

Immunogen

Fusion protein.
Epitope: human

Analysis Note

Control
Positive Control: WI-38 (ATCC# CCL75) or U251 cell lines

Application

Immunoblotting: 1:1,000-1:2,000 on denatured protein. Western blot: 1:1000-1:5000. Recognizes a 220-240kDA band on denaturing SDS gels. There is also a 60-64kDa band present in most lysates, the identity of this smaller fragment is unknown but it is not seen in lysates not containing human nestin protein.

WI-38 (ATCC# CCL75) can be used as a positive control. U251 cell line is also positive.

Immunocytochemistry: 1:200 on cells fixed in 4% paraformaldehyde and permeabilized with 0.02% Triton

Immunohistochemistry: paraformaldehyde is the preferred fixative: 1:200 on formalin fixed, paraffin embedded tissue sections. Use triton 0.02% in block only; dilution buffer 0.1% BSA; incubations 1-2 hours RT. Human fetal brain is suggested as a positive control. (CA Messam et al, Develop. Brain Res, 134:87-92, 2002; Gu, et al., Brain Res. 943:174-180, 2002). Filamentous staining in the cytoplasm of cells

Optimal working dilutions must be determined by end user.
Research Category
Stem Cell Research
Research Sub Category
Neural Stem Cells
This Anti-Nestin Antibody, clone 10C2 is validated for use in IC, IH, IH(P), WB for the detection of Nestin.

Biochem/physiol Actions

Reacts with human Nestin, with weak reactivity to monkey nestin. Not reactive with rodent nestin.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

General description

200-220 kDa
Nestin, a large intermediate filament protein (class Type VI) expressed during development and in myotendinous and neuromuscular junctions. Nestin expression is restricted, typically disappearing by E18. Nestin is thought to be a reasonable neuronal marker; however, recent studies have found nestin expression in other cell types such as endothelial cells {Folia Biologica, 44(5):155, 1998}. Nestin identifies the most primitive neuroepithelium but also identifies many other embryonic tissues, so it is not specific for CNS. Nestin expression is seen in almost all GBMs (Glioblastoma mulitformes) and many melanomas (both primary and metastatic) but not in any metastatic carcinoma.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Replaces: AB5922

Physical form

Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified

Preparation Note

Antibodies may be stored at 4°C or -20°C. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For products with volumes of 200μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container′s cap.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

저장 등급

12 - Non Combustible Liquids

wgk

WGK 2

시험 성적서(COA)

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문서 라이브러리 방문

beta-cell neogenesis induced by adenovirus-mediated gene delivery of transcription factor pdx-1 into mouse pancreas.
Taniguchi, H, et al.
Gene Therapy, 10, 15-23 (2003)
Cannabinoids induce glioma stem-like cell differentiation and inhibit gliomagenesis.
Aguado, Tania, et al.
The Journal of Biological Chemistry, 282, 6854-6862 (2007)
A novel myoepithelial/progenitor cell marker in the breast?
Zdenek Kolar,Jiri Ehrmann,Gulisa Turashvili,Jan Bouchal,Jaroslav Mokry
Virchows Archiv null
Neurogenic neuroepithelial and radial glial cells generated from six human embryonic stem cell lines in serum-free suspension and adherent cultures.
Roxana Nat, Mats Nilbratt, Susanna Narkilahti, Bengt Winblad, Outi Hovatta, Agneta Nordberg
Glia null
C A Messam et al.
Experimental neurology, 161(2), 585-596 (2000-02-25)
The presence of the intermediate filament protein nestin has been the predominant marker used to describe stem and progenitor cells in the mammalian CNS. In this study, a 998-bp fragment in the 3' region of the nestin mRNA was cloned
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A stem cell culture protocol to generate 3D NSC models of Alzheimer’s disease using ReNcell human neural stem cell lines.

Neural Stem Cell Culture Protocols

Step-by-step culture protocols for neural stem cell culture including NSC isolation, expansion, differentiation and characterization.

관련 콘텐츠

Human Stem Cell Systems

As the focus of stem cell research undergoes a transition from animal to human models, researchers are in critical need of validated products to support the isolation, maintenance, differentiation, and characterization of human stem cells. While many reagents designed for rodent systems can be applied to human stem cell studies, they are not truly optimized for robust human stem cell culture or analysis. This is why human stem cell researchers have always trusted EMD Millipore, the first provider of commercially available human embryonic stem cells and human neural stem cell lines, to accelerate their research. All of our human stem cell systems are extensively tested in defined media culture, and differentiated progeny are comprehensively characterized with highly validated antibodies and detection reagents.

PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD
Reprogramming Cell Fate and Function Novel Strategies for iPSC Generation, Characterization, and Differentiation

국제 무역 품목 번호

SKUGTIN
MAB532608436037124167
MAB5326-25UG04054839337505

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