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MABS2185-25UL Anti-NPHP1 Antibody, clone 1M4

Anti-NPHP1, clone 1M4, Cat. No. MABS2185, is a mouse monoclonal antibody that detects Nephrocystin-1 and has been tested for use in Immunofluorescence and Western Blotting.

MABS2185-25UL
25 µL  
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      개요

      Replacement Information
      Description
      Catalogue NumberMABS2185-25UL
      DescriptionAnti-NPHP1 Antibody, clone 1M4
      Alternate Names
      • Nephrocystin-1
      • Juvenile nephronophthisis 1 protein
      Background InformationNephrocystin-1 (UniProt: O15259; also known as Juvenile nephronophthisis 1 protein) is encoded by the NPHP1 (also known as NPH1) gene (Gene ID: 4867) in human. Nephrocystin-1 displays a widespread expression with highest levels found in pituitary gland, spinal cord, thyroid gland, testis, lymph node, trachea and skeletal muscle. Low expression has been reported in heart, kidney, and pancreas. It serves as a cytoplasmic adaptor molecule and contains a putative coiled-coil domain and an SH3 domain (aa 152-212), which mediates its stable interaction with p130Cas (BCAR1). Its SH3 domain also serves as a potential binding partner of polycystin-1. Nephrocystin-1 is shown to localize to cell-cell junctions, cilia, and cell-matrix adhesion sites and together with p130Cas it plays a role in the control of epithelial cell polarity. It is also involved in the organization of apical junctions in kidney cells along with nephrocystin-4 and -8. In the retinal photoreceptor cell layer, nephrocystin-1 is shown to be localized at the connecting cilium. It can undergo phosphorylation at serine 121, 123, and 126 by casein kinase 2 and this phosphorylation is required for the interaction with phosphofurin acidic cluster sorting protein 1 (PACS-1) and the targeting to the base region of cilia. Mutations in NPHP1 gene are known to cause Nephronophthisis 1, a early age disease characterized by anemia, polyuria, polydipsia, and death in uremia due to symmetrical destruction of kidneys involving both tubules and glomeruli. (Ref.: Wodarczyk, C., et al. (2010). PLoS One. 5(9); e12719).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG3 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-NPHP1, clone 1M4, Cat. No. MABS2185, is a mouse monoclonal antibody that detects Nephrocystin-1 and has been tested for use in Immunofluorescence and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      Application NotesImmunofluorescence Analysis: A representative lot of this antibody detected NPHP1 in Immunofluorescence applications (Xu, Q., et. al. (2016). Nat Commun. 7:10777).
      Biological Information
      ImmunogenA synthetic polypeptide fused by 16 different NPHP1 peptide sequences predicted to have the highest antigenicity. The sequence was converted to cDNA and the polypeptide was expressed and purified from E. coli.
      Clone1M4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 1M4 is a mouse monoclonal antibody that specifically detects human Nephrocystin-1 (NPHP1).
      IsotypeIgG3κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NPHP1
      • NPH1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~92 kDa observed; 83.30 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in human testis tissue lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected NPHP1 in human testis tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABS2185-25UL 04061841134848

      Documentation

      Anti-NPHP1 Antibody, clone 1M4 MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-NPHP1 Antibody, clone 1M4 Certificates of Analysis

      TitleLot Number
      Anti-NPHP1, clone 1M4 - 3327012 3327012

      References

      Reference overviewPub Med ID
      Phosphatidylinositol phosphate kinase PIPKIγ and phosphatase INPP5E coordinate initiation of ciliogenesis.
      Xu, Q; Zhang, Y; Wei, Q; Huang, Y; Hu, J; Ling, K
      Nat Commun  7  10777  2016

      요약 표시       		26916822 26916822