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AB1964 Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485]

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AB1964
100 µL  
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      개요

      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, RELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1964
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-N-WASP Antibody, phospho-specific [Ser484/Ser485]
      Alternate Names
      • Neural Wiskott-Aldrich Syndrome Protein
      Background InformationMembers of the Wiskott-Aldrich sydrome protein (WASP) family regulate the formation of actin-based cell structures in many cell types. These proteins contain C-terminal actin-binding domains that can stimulate actin polymerization. In addition, these proteins bind the ARP2/3 complex, which can nucleate actin polymerization at sites that lead to branched actin structures. WASP is expressed primarily in hematopoietic cells, while its homolog N-WASP is widely expressed. These proteins have 48% identity in human with the highest homology in the functional regions of these proteins. Phosphorylation regulates the activity of both proteins. Dual phosphorylation of WASP on serine 483 and 484 by casein kinases increase the affinity for the ARP2/3 complex. Thus, dual serine phosphorylation may be important for formation of actin-based structures in various cell types.
      Product Information
      FormatAffinity Purified
      PresentationThis antibody was cross-adsorbed to unphosphorylated-N-WASP (S484/S485) peptide (without carrier) then affinity purified using phospho-N-WASP (S484/S485) peptide (without carrier). Antibody is supplied in 100 μl phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationDetect N-WASP using this Anti-N-WASP Antibody, phospho-specific [Ser484/Ser485] validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1:1000 ELISA: 1:2000
      Biological Information
      ImmunogenPhospho-N-WASP (S484/S485) synthetic peptide (coupled to KLH) corresponding to amino acid residues around serine 484 and 485 of human N-WASP. The human WASP sequence has a similar peptide sequence surrounding serine 483 and 484.
      Epitopephosphoserine484/phosphserine485
      HostRabbit
      SpecificityThe antibody detects a 63 kDa protein corresponding to the molecular weight of phosphorylated WASP on SDS-PAGE immunoblots of Jurkat cell lysate. In rat brain, A431, human endothelial and SKN-SH cells, this antibody detects a 65 kDa protein corresponding to phosphorylated N-WASP.
      IsotypeIgG
      Species Reactivity
      • Human
      • Rat
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe Wiskott-Aldrich syndrome (WAS) family of proteins share similar domain structure, and are involved in transduction of signals from receptors on the cell surface to the actin cytoskeleton. The presence of a number of different motifs suggests that they are regulated by a number of different stimuli, and interact with multiple proteins. Recent studies have demonstrated that these proteins, directly or indirectly, associate with the small GTPase, Cdc42, known to regulate formation of actin filaments, and the cytoskeletal organizing complex, Arp2/3. The WASL gene product is a homolog of WAS protein, however, unlike the latter, it is ubiquitously expressed and shows highest expression in neural tissues. It has been shown to bind Cdc42 directly, and induce formation of long actin microspikes.
      Gene Symbol
      • WASL
      • DKFZp779G0847
      • MGC48327
      • N-WASP
      • NWASP
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O00401 # Regulates actin polymerization by stimulating the actin- nucleating activity of the Arp2/3 complex. Binds to HSF1/HSTF1 and forms a complex on heat shock promoter elements (HSE) that negatively regulates HSP90 expression.
      SIZE: 505 amino acids; 54827 Da
      SUBUNIT: Binds actin and the Arp2/3 complex. Interacts with CDC42. Binds to SH3 domains of GRB2. Interacts with the C-terminal SH3 domain of DNMBP. Interacts with the WW domains of PRPF40A/FBP11 (By similarity). Interacts with NOSTRIN. Interacts with Shigella flexneri protein icsA. The interaction with icsA enhances the affinity of WASL for Arp2/3, thus assembling a tight complex which has maximal activity in actin assembly.
      SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton (By similarity). Nucleus (By similarity). Note=Preferentially localized in the cytoplasm when phosphorylated and in the nucleus when unphosphorylated (By similarity).
      SIMILARITY: SwissProt: O00401 ## Contains 1 CRIB domain. & Contains 1 WH1 domain. & Contains 2 WH2 domains.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 20°C for up to one year from date of receipt. Do not aliquot.
      Packaging Information
      Material Size100 µL
      Global Trade Item Number
      카탈로그 번호 GTIN
      AB1964 04053252514357