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IM09L Anti-MMP-9 (Ab-1) Mouse mAb (6-6B)

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IM09L
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개요

주요 사양표

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

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IM09L-100UGCN
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      Plastic ampoule 100 μg
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      Description
      OverviewRecognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 under non-reducing conditions, but only the latent form under reducing conditions. Inhibits the enzymatic activity of MMP-9. For paraffin sections, use Cat. No. IM37L.
      Catalogue NumberIM09L
      Brand Family Calbiochem®
      SynonymsAnti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9, Anti-Type IV Collagenase, Anti-Gelatinase B
      References
      ReferencesCottam, D.W. and Rees, R.C. 1993. Intl. J. of Oncol. 2, 861.
      Nakajima, M., et al. 1993. Cancer Res. 53, 5802.
      Ramos-DeSimone, et al. 1993. Hybridoma. 12, 349.
      Stetler-Stevenson, W.G., et al. 1993. FASEB. 7, 1434.
      Zucker, S., et al. 1993. Cancer Res. 53, 140.
      Woessner, J.F. 1991. FASEB. 5, 2145.
      Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1; 99-106.
      Product Information
      DeclarationNot available for sale in Japan.
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Positive controlMMP-9 protein (Cat. Nos. PF024 or PF038)
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation Ramos-DeSimone, N. and French, D. L. 1994. Ann. NY Acad. Sci. 732, 469. Immunoblotting, Neutralization Studies Ramos-DeSimone, N., et al. 1993. Hybridoma. 12, 349.
      Key Applications Immunoblotting (Western Blotting)
      Immunoprecipitation
      Not Paraffin Sections
      Neutralization Studies
      Application NotesImmunoblotting (2 µg/ml)
      Immunoprecipitation (see appplication references)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (not recommended)
      Application CommentsInhibits MMP-9 enzymatic activity. For staining paraffin sections, use Anti-MMP-9 (626-644) (Ab-3) Mouse mAb (56-2A4) (Cat. No. IM37L). Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenMMP-9 from conditioned medium of PMA-stimulated HT-1080 human fibrosarcoma cells
      ImmunogenHuman
      Clone6-6B
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsStore at 4°C until reconstituted, then store in aliquots at -20°C or at 4°C with 0.1% azide. Resuspend the antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline, pH 7.4, to yield a final concentration of 100 µg/ml; product will be more stable if 0.1% sodium azide is included (do not add azide if antibody is to be used with viable cells). Lyophilized antibodies should be resuspended at 4°C with occasional gentle mixing for at least 2 h. Freezing of aliquots is best for long-term storage of reconstituted product; repetitive freezing and thawing should be avoided.
      Global Trade Item Number
      카탈로그 번호 GTIN
      IM09L-100UGCN 04055977228151