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05-157-I-25UL Anti-MAPK2 (ERK2) Antibody, clone 1B3B9

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05-157-I-25UL
25 µL  
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      개요

      Replacement Information
      Description
      Catalogue Number05-157-I-25UL
      DescriptionAnti-MAPK2 (ERK2) Antibody, clone 1B3B9
      Alternate Names
      • Mitogen-activated protein kinase 1
      • EC:2.7.11.24
      • MAP kinase 1
      • MAPK 1
      • ERT1
      • Extracellular signal-regulated kinase 2
      • ERK-2
      • MAP kinase isoform p42
      • p42-MAPK
      • Mitogen-activated protein kinase 2
      • MAP kinase 2
      Background InformationMitogen-activated protein kinase 1 (UniProt: P63085; also known as EC:2.7.11.24; also known as MAP kinase 1, MAPK 1, ERT1, Extracellular signal-regulated kinase 2, ERK-2, MAP kinase isoform p42, p42-MAPK, Mitogen-activated protein kinase 2, MAP kinase 2) is encoded by the MAPK1 (also known as ERK2, PRKM1, PRKM2) gene (Gene ID: 26413) in murine species. ERK-2 is a widely expressed proline-directed serine/threonine protein kinase that is an essential component of the MAP kinase signal transduction pathway. Its protein kinase domain is localized to amino acids 23-311. ERK-2 binds both upstream activators and downstream substrates in multimolecular complexes. Its substrates include multiple transcription factors, cytoskeletal elements, regulators of apoptosis, and regulators of translation, and signaling molecules. MAPK/ERK cascade mediates diverse biological functions such as cell growth, adhesion, survival and differentiation through the regulation of transcription, translation, cytoskeletal rearrangements. ERK-2 is phosphorylated by MEK1 and MEK2 on threonine 183 and tyrosine 185 in the conserved TEY (Thr-Glu-Tyr) motif in response to external stimuli like insulin or NGF. Both phosphorylations are required for its activity. This phosphorylation causes conformational changes that enable full activation and interaction with its substrates.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-MAPK2 (ERK2), clone 1B3B9, Cat. No. 05-157-I, is a mouse monoclonal antibody that detects MAPK2 (ERK2) and is tested for use in Immunoprecipitation and Western Blotting.
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      Application NotesImmunoprecipitation Analysis: A representative lot immunoprecipitated MAPK2 (ERK2) in Immunoprecipitation applications (Kumar, N.V., et. al. (2001). J Biol Chem. 276(34):32362-72).

      Western Blotting Analysis: A representative lot detected MAPK2 (ERK2) in Western blotting applications (Sato, Y., et. al. (2001). J Biol Chem.276(15):11956-62; Wu, C., et. al. (2001). J Neurosci. 21(15):5406-16; Yamaguchi, Y., et. al. (2001). J Biol Chem. 276(22):18977-83; Utani, A., et. al. (2003). J Biol Chem. 278(36):34483-90).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHPLC purified recombinant mouse p42MAP kinase (ERK2).
      EpitopeUnknown
      Clone1B3B9
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone 1B3B9 is a mouse monoclonal antibody that recognizes MAPK2.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      • Rat
      • Mouse
      Species Reactivity NoteHuman, Mouse, Rat.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • MAPK1
      • ERK2
      • PRKM1
      • PRKM2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~40 kDa observed; 41.28 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A431 cell lysate.

      Western Blotting Analysis: A 1:500 dilution of this antibody detected MAPK2 (ERK2) in A431 cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      05-157-I-25UL 04061842592012

      Documentation

      Anti-MAPK2 (ERK2) Antibody, clone 1B3B9 MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-MAPK2 (ERK2) Antibody, clone 1B3B9 Certificates of Analysis

      TitleLot Number
      Anti-MAPK2 (ERK2), clone 1B3B9 - Q3504043 Q3504043

      References

      Reference overviewApplicationPub Med ID
      Laminin alpha 3 LG4 module induces matrix metalloproteinase-1 through mitogen-activated protein kinase signaling
      Atsushi Utani 1 , Yutaka Momota, Hideharu Endo, Yoshitoshi Kasuya, Konrad Beck, Nobuharu Suzuki, Motoyoshi Nomizu, Hiroshi Shinkai
      J Biol Chem  278(36)  34483-90  2003

      요약 표시
      Immunoblotting (Western)12826666 12826666
      Overexpression of N-acetylglucosaminyltransferase III enhances the epidermal growth factor-induced phosphorylation of ERK in HeLaS3 cells by up-regulation of the internalization rate of the receptors
      Y Sato 1 , M Takahashi, Y Shibukawa, S K Jain, R Hamaoka, Miyagawa Ji, Y Yaginuma, K Honke, M Ishikawa, N Taniguchi
      J Biol Chem  276(15)  11956-62  2001

      요약 표시
      Immunoblotting (Western)11134020 11134020
      RhoA inhibits the nerve growth factor-induced Rac1 activation through Rho-associated kinase-dependent pathway
      Y Yamaguchi 1 , H Katoh, H Yasui, K Mori, M Negishi
      J Biol Chem  276(22)  18977-83  2001

      요약 표시
      Immunoblotting (Western)11279039 11279039
      Nerve growth factor activates persistent Rap1 signaling in endosomes
      C Wu 1 , C F Lai, W C Mobley
      J Neurosci  21(15)  5406-16  2001

      요약 표시
      Immunoblotting (Western), Immunoprecipitation11466412 11466412
      Ten ERK-related proteins in three distinct classes associate with AP-1 proteins and/or AP-1 DNA
      N V Kumar 1 , L R Bernstein
      J Biol Chem  276(34)  32362-72  2001

      요약 표시
      Kinase Assay11431474 11431474