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MABS814 Anti-MAP2K4, clone 5H4 Antibody

Anti-MAP2K4, clone 5H4 Antibody is an antibody against MAP2K4 for use in Western Blotting, Immunohistochemistry, Flow Cytometry, ELISA.

Anti-MAP2K4, clone 5H4 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Developmental Signaling Gene Symbol: MAP2K4, JNKK1, MEK4, MKK4, PRKMK4, SEK1, SERK1, SKK1 UniProt Number: P45985
MABS814
100 µL   
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      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IHC, FC, ELISAMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABS814
      Replaces04-382
      DescriptionAnti-MAP2K4, clone 5H4 Antibody
      Alternate Names
      • Dual specificity mitogen-activated protein kinase kinase 4
      • MAP kinase kinase 4
      • MAPKK 4
      • MAP2K4
      Background InformationDual specificity mitogen-activated protein kinase kinase 4 (MAP2K4) is a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
      Quality LevelMQ300
      Applications
      ApplicationAnti-MAP2K4, clone 5H4 Antibody is an antibody against MAP2K4 for use in Western Blotting, Immunohistochemistry, Flow Cytometry, ELISA.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      • Flow Cytometry
      • ELISA
      Application NotesImmunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected MAP2K4 in muscle tissue.

      Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected MAP2K4 in K562 cells.

      ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
      Biological Information
      ImmunogenPurified recombinant fragment of human MAP2K4 expressed in E. Coli.
      Clone5H4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • MAP2K4
      • JNKK1
      • MEK4
      • MKK4
      • PRKMK4
      • SEK1
      • SERK1
      • SKK1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~40 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HepG2, K562 and HEK293 cell lysates.

      Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K4 in HepG2, K562 and HEK293 cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABS814 04055977172324