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ABE1367-100UG Anti-LCoR (Splice Variant)

Anti-LCoR, Cat. No. ABE1367, is a highly specific rabbit polyclonal antibody that targets a splice variant form of Ligand-dependent corepressor (LCoR) and has been tested in Immunocytochemistry and Western Blotting.

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ABE1367-100UG
100 μg  
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      개요

      Replacement Information
      Description
      Catalogue NumberABE1367-100UG
      DescriptionAnti-LCoR (Splice Variant)
      Alternate Names
      • Ligand-dependent corepressor
      • Mblk1-related protein 2
      • C10ORF12
      • LCoR (splice variant)
      • PALI1
      Background InformationLigand-dependent corepressor (UniProt: Q96JN0; also known as LCoR, Mblk1-related protein 2) is encoded by the LCOR (also known as C10orf12, KIAA1795, MLR2) gene (Gene ID: 84458) in human. LCoR is a ubiquitously distributed protein in fetal and adult tissues and serves as a transcriptional co-repressor that is recruited to agonist-bound nuclear receptors through a single LxxLL motif at the N-terminal region. It may also act as a transcription activator that binds DNA elements with the sequence 5'-CCCTATCGATCGATCTCTACCT-3'. Three isoforms of LCoR have been described that are produced by alternative splicing. This polyclonal antibody is raised against a large isoform (isoform 3; a splice variant), which associates with the PRC2 complex through its C-terminal region. Its nuclear localization signal is reported to be in amino acids 339-345 and it reacts with nuclear receptors via amino acids 53-57. (Ref.: Conway, E., et al. (2018). Mol. Cell 70(3); 408-421).
      References
      Product Information
      FormatPurified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-LCoR, Cat. No. ABE1367, is a highly specific rabbit polyclonal antibody that targets a splice variant form of Ligand-dependent corepressor (LCoR) and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • Western Blotting
      Application NotesWestern Blotting Analysis: A 1:500 dilution from a representative lot detected LCoR in whole cell lysates from Pali1 KO and matched wild type mouse embryonic stem (Courtesy of Dr. Eric Conway, Dr. Emilia Jerman & Prof. Adrian P. Bracken at the Smurfit Institute of Genetics, Trinity College Dublin, Ireland).

      Western Blotting Analysis: A representative lot detected LCoR in Western Blotting applications (Conway, E., et. al. (2018). Mol. Cell. 70(3):408-421).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 20 amino acids from the N-terminal half of human Ligand-dependent corepressor (LCoR), isoform 3.
      Epitopeunknown
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects a splice variant of human and murine Ligand-dependent corepressor (LCoR). It targets an epitope with in the N-terminal half.
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LCOR
      • C10orf12
      • KIAA1795
      • MLR2
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~250 kDa observed; 170.48 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in NTERA2 cells.

      Immunocytochemistry Analysis: A 1:50 dilution of this antibody detected LCoR in NTERA2 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      ABE1367-100UG 04054839757525

      Documentation

      Anti-LCoR (Splice Variant) Certificates of Analysis

      TitleLot Number
      Anti-LCoR (Splice Variant) - 3455243 3455243
      Anti-LCoR (Splice Variant) - 3910139 3910139
      Anti-LCoR (Splice Variant) - Q3207404 Q3207404

      References

      Reference overviewPub Med ID
      A Family of Vertebrate-Specific Polycombs Encoded by the LCOR/LCORL Genes Balance PRC2 Subtype Activities.
      Conway, E; Jerman, E; Healy, E; Ito, S; Holoch, D; Oliviero, G; Deevy, O; Glancy, E; Fitzpatrick, DJ; Mucha, M; Watson, A; Rice, AM; Chammas, P; Huang, C; Pratt-Kelly, I; Koseki, Y; Nakayama, M; Ishikura, T; Streubel, G; Wynne, K; Hokamp, K; McLysaght, A; Ciferri, C; Di Croce, L; Cagney, G; Margueron, R; Koseki, H; Bracken, AP
      Mol Cell  70  408-421.e8  2018

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