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MABT1460-25UL Anti-LAMC2 Antibody, clone P2H

Anti-LAMC2, clone P2H, Cat. No. MABT1460, is a mouse monoclonal antibody that detects Laminin subunit g2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.

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MABT1460-25UL
25 µL  
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      개요

      Replacement Information
      Description
      Catalogue NumberMABT1460-25UL
      DescriptionAnti-LAMC2 Antibody, clone P2H
      Alternate Names
      • Laminin subunit gamma-2
      • Cell-scattering factor 140 kDa subunit
      • CSF 140 kDa subunit
      • Epiligrin subunit gamma
      • Kalinin subunit gamma
      • Kalinin/nicein/epiligrin 100 kDa subunit
      • Ladsin 140 kDa subunit
      • Laminin B2t chain
      • Laminin-5 subunit gamma
      • Large adhesive scatter factor 140 kDa subunit
      • Nicein subunit gamma
      Background InformationLaminin subunit g2 (UniProt: Q13753; also known as Cell-scattering factor 140 kDa subunit, CSF 140 kDa subunit, Epiligrin subunit gamma, Kalinin subunit gamma, Kalinin/nicein/epiligrin 100 kDa subunit, Ladsin 140 kDa subunit, Laminin B2t chain, Laminin-5 subunit gamma, Large adhesive scatter factor 140 kDa subunit, Nicein subunit gamma) is encoded by the LAMC2 (also known as LAMB2T, LAMNB2) gene (Gene ID: 3918) in human. Laminin is a major basement membrane glycoprotein consisting of α, β, γ chains that are bound to each other by disulfide bonds. Over 15 different isoforms have been reported in the laminin family that are expressed in a tissue-specific manner and play different role in each tissue. γ² a subunit of Laminin-5 is reported to be frequently over-expressed as a monomer or as the β³-γ² heterodimer in invasive cancers and is regarded as an invasion marker. It has 8 EGF-like domains. The cell adhesion and motility activities of Laminin-5 are reported to be regulated by the proteolytic cleavage of the short arm of Laminin γ² chain by BMP-1 or MT1-MMP. This cleavage releases a 45 kDa N-terminal proteolytic fragment (γ²pf). The short arm and γ²pf can be further cleaved by serine proteases and by matrix metalloproteinases resulting in the release of small N-terminal fragments. γ²pf‐like fragments have been reported to be present in conditioned media of cultured cancer cells and in sera from human cancer subjects. Clone P2H targets the second N-terminal EGF-like repeat (NE2) of domain V of laminin 2. (Ref.: Sato, H., et al. (2014). Cancer Sci. 105(2); 168-175; Miyazaki, K., et al. (2016). Cancer Sci. 107(12); 1909-1918).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ200
      Applications
      ApplicationAnti-LAMC2, clone P2H, Cat. No. MABT1460, is a mouse monoclonal antibody that detects Laminin subunit g2 and is tested for use in ELISA, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
      Key Applications
      • Immunocytochemistry
      • ELISA
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern Blotting Analysis: 1 µg/mL from a representative lot detected LAMC2 in lung squamous cell carcinoma cell lysates (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

      Western Blotting Analysis: A representative lot detected LAMC2 in Western Blotting applications (Miyazaki, K., et. al. (2016). Cancer Sci. 107(12):1909-1918).

      Immunohistochemistry (Paraffin) Analysis: 10 µg/mL from a representative lot detected LAMC2 in normal human skin, skin cancer, normal breast, and breast adenocarcinoma tissues (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

      ELISA Analysis: 5 µg/mL from a representative lot detected LAMC2 in Lm-gamma2pf and Lm-gamma2 in Lm332 condition medium from Lm332-expressing HEK293 cells (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

      Immunocytochemistry Analysis: A representative lot detected LAMC2 in Immunocytochemistry applications (Miyazaki, K., et. al. (2016). Cancer Sci. 107(12):1909-1918).

      Immunohistochemistry Applications: A representative lot detected LAMC2 in Immunohistochemistry applications of lung squamous cell carcinomas and lung adenocarcinomas (Courtesy of Kaoru Miyazaki, Ph.D., KIhara Institute for Biological Research, Yokohama City University, Japan).

      Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
      Biological Information
      ImmunogenHis-tagged 45-kDa N-terminal fragment consisting of g2pf, domain V.
      EpitopeN-terminal
      CloneP2H
      Concentration0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.
      HostMouse
      SpecificityClone P2H is a mouse monoclonal antibody that detects Laminin-5, g2 chain. It targets an epitope within the N-terminal, NE2 in domain V.
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NoteHuman, Mouse.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • LAMC2
      • LAMB2T
      • LAMNB2
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight130.98 kDa calculated for Laminin-5, g2 chain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:500 dilution of this antibody detected Laminin-5 subunit g2 (LAMC2) in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at +2°C to +8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABT1460-25UL 04065265872669