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MABN2619-25UL Anti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12

Anti-Kininogen-1, cleaved form, clone 4B12, Cat. No. MABN2619, is a mouse monoclonal antibody that detects Kininogen-1 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Immunoprecipitation.

MABN2619-25UL
25 µL  
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      개요

      Replacement Information
      Description
      Catalogue NumberMABN2619-25UL
      DescriptionAnti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12
      Alternate Names
      • Alpha-2-thiol proteinase inhibitor
      • Fitzgerald factor
      • High molecular weight kininogen
      • HMWK
      • Williams-Fitzgerald-Flaujeac factor
      Background InformationKininogen-1 (UniProt: P01042; also known as Alpha-2-thiol proteinase inhibitor, Fitzgerald factor, High molecular weight kininogen, HMWK, Williams-Fitzgerald-Flaujeac factor, KNG) is encoded by the KNG1 (also known as BDK, KNG) gene (Gene ID: 3827) in human. Kininogen (KNG) is the precursor protein of the kallikrein-kinin system. KNGs are shown to act as inhibitors of proteases. Kininigen-1 is a secreted protein that is synthesized with a signal peptide (aa 1-18), which is subsequently cleaved off to generate the mature form that can be further cleave into Kininigen-1 heavy chain (HMW; aa 19-380), Kininien-1 light chain (LMW; aa 390-644), T-kinin (aa 376-389), Bradykinin (aa 381-389), lysyl-bradykinin (aa 380-389), and Low molecular weight growth-promoting factor (aa 431-434). Kininogen-1 is reported to exert anti-angiogenic properties and block the proliferation of endothelial cells. It has been identified as a serum biomarker for advanced colorectal adenoma and colorectal cancer. The HMW-kininogen plays a role in blood coagulation by helping to position prekallikrein and factor XI next to factor XII. It is shown to inhibit the thrombin- and plasmin-induced aggregation of thrombocytes. Activation of factor XII triggers HMW-kininogen cleavage by plasma kallikrein, which results in the generation of cleaved high molecular-weight kininogen (HKc) and the release of bradykinin. Higher rate of HMW-kininogen and elevated levels of plasma kallikrein activity has been recorded in the plasma of Alzheimer s disease subjects and changes HMW and cleaved kininogen levels have been significantly correlated with dementia and neuritic plaque scores. Clone 4B12 detects only the cleaved form (LMW) of kininogen (HKc) and not the HMW form (HKi). (Ref.: Yamamoto-Imoto, H., et al (2018). Alzheimer. Dement. (Amst). 10; 480-489; Xu, J., et al. (2018). J. Exp. Clin. Cancer Res. 37; Article 180).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Kininogen-1, cleaved form, clone 4B12, Cat. No. MABN2619, is a mouse monoclonal antibody that detects Kininogen-1 and is tested for use in ELISA, Immunohistochemistry (Paraffin), and Immunoprecipitation.
      Key Applications
      • ELISA
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application NotesEnzyme Immunoassay (ELISA) Analysis: A representative lot detected Kininogen-1 in ELISA applications (Yamamoto-Imoto, H., et. al. (2018). Alzheimers Dement (Amst). 10:480-489).

      Immunoprecipitation Analysis: A representative lot immunoprecipitated Kininogen-1 in Immunoprecipitation applications (Yamamoto-Imoto, H., et. al. (2018). Alzheimers Dement (Amst). 10:480-489).
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 19 amino acids from the C-terminal region of human Kininogen-1.
      Clone4b12
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4B12 is a mouse monoclonal antibody that detects human Kininogen-1. It targets an epitope within 19 amino acids from the C-terminal region. It recognizes only the cleaved form.
      IsotypeIgG1κ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • KNG1
      • BDK
      • KNG
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight71.96 kDa calculated.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunohistochemistry (Paraffin) in human kidney tissue sections.

      Immunohistochemistry (Paraffin) Analysis: A 1:50 dilution of this antibody detected Kininogen-1 in human kidney tissue sections.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABN2619-25UL 04061841440772

      Documentation

      Anti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12 MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-Kininogen-1 Antibody, cleaved form Antibody, clone 4B12 Certificates of Analysis

      TitleLot Number
      Anti-Kininogen-1, cleaved form, clone 4B12 - Q3416292 Q3416292

      References

      Reference overviewPub Med ID
      A novel detection method of cleaved plasma high-molecular-weight kininogen reveals its correlation with Alzheimer's pathology and cognitive impairment.
      Yamamoto-Imoto, H; Zamolodchikov, D; Chen, ZL; Bourne, SL; Rizvi, S; Singh, P; Norris, EH; Weis-Garcia, F; Strickland, S
      Alzheimers Dement (Amst)  10  480-489  2018

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