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AB2215 Anti-Interferon-β Antibody

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AB2215
100 µL  2x10(e4) units
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      개요

      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MELISA, IP, WB, IHC, NEUTRbSerumPolyclonal Antibody
      Description
      Catalogue NumberAB2215
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Interferon-β Antibody
      Product Information
      FormatSerum
      PresentationRabbit polyclonal serum in buffer containing 0.3 M NaHCO3 and 200 mM NaCl.
      Quality LevelMQ100
      Applications
      ApplicationDetect Interferon-β using this Anti-Interferon-β Antibody validated for use in ELISA, IP, WB, IH, NEUT.
      Key Applications
      • ELISA
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      • Neutralizing
      Application NotesCytopathic effect inhibition assay: 2 x 10E5 neutralization units per mL of antiserum. One neutralization unit is the amount of antiserum required to neutralize 1 unit of mouse IFN-beta. This material is prepared specifically for effective neutralization of mouse IFN-beta.

      Western blot (1:500) The antibody reacts with a 19kDa band coresponding to Mu-IFN-beta. Protocol below:

      1. Put protein (approx 50ng) in blocking solution for 30-60 min RT

      2. Add Primary antibody (1:500) to blocking solution

      3. Agitate membrane for 60 min, RT

      4. Wash 3X for 5min each with PBS-Tween

      5. Add secondary antibody (1:1000) after diluting in PBS-Tween

      6. Gentle agitation for 60min.

      7. Wash 3X for 5min each with PBS-Tween

      8. Develop with reaction kit of choice

      Immunoprecipitation

      Immunohistochemistry

      ELISA

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenMouse IgG, whole molecule.
      HostRabbit
      SpecificityReacts with mouse Interferon beta (IFNbeta). No reactivity is seen with IFNalpha or gamma
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • IFNB1
      • IFF
      • MGC96956
      • IFN-beta
      • IFB
      • IFNB
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P01574 # Has antiviral, antibacterial and anticancer activities.
      SIZE: 187 amino acids; 22294 Da
      SUBUNIT: Monomer.
      SUBCELLULAR LOCATION: Secreted.
      SIMILARITY: SwissProt: P01574 ## Belongs to the alpha/beta interferon family.
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C or below for retention of full activity, for up to 12 months. When thawing, the contents of the tube should be apportioned in separate tubes so that freezing and thawing is kept to a minimum.
      Packaging Information
      Material Size100 µL
      Material Package2x10(e4) units
      Global Trade Item Number
      카탈로그 번호 GTIN
      AB2215 04053252327322