Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31

IGF-1R/3T3 mouse fibroblasts transfected with human type I IGF-receptor cDNA.

Acts as a Weak IGF-like Agonist in Bioassays (Thymidine incorporation into DNA)

Immunoprecipitation (Use Protein G): 2μg antibody/mg protein lysate.

Immunohistochemistry (Frozen and formalin/paraffin): Use antibody at 2-4ug/mL for 30 min. at room temperature. Staining of formalin-fixed tissues requires boiling tissue sections in 1 mM EDTA, pH 8.0, for 10-20 min. followed by cooling at room temperature for 20 minutes. Please note that EDTA is better than citrate for epitope unmasking.

Optimal working dilutions must be determined by end user.

Anti-Insulin-like Growth Factor-1 Receptor Antibody, α-subunit, clone 24-31 detects level of Insulin-like Growth Factor-1 Receptor & has been published & validated for use in IH(P) & IP.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Recognizes the alpha-subunit of type I Insulin-like Growth Factor Receptor (IGF-1R). It shows no cross-reaction with insulin receptor. Its epitope localizes between a.a. 283-440 (exon 4-6) of IGF-1R. Both IGF-1R and insulin receptor are synthesized as a single polypeptide which is glycosylated and proteolytically cleaved to give the alpha- and beta-subunits, which are disulfide-linked in a beta-alpha-alpha-beta configuration in the mature receptor. The a-subunit is completely extracellular, while the beta-subunit spans the membrane and the intracellular portion has intrinsic tyrosine kinase activity. It is assumed that, as for the insulin receptor, the tyrosine kinase activity is essential for IGF-1 action.

Weakly reacts with Rabbit.

Format: Purified

Purified from ascites fluid by Protein G chromatography. Liquid in 10mM PBS, pH 7.4, with 0.2% BSA and 0.09% sodium azide.

Maintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.

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