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MABE1119 Anti-H2AK15ub Antibody, clone EDL H2AK15-4

Anti-H2AK15ub, clone EDL H2AK15-4, Cat. No. MABE1119, is a highly specific mouse monoclonal antibody, that targets H2A ubiquitylated at Lys15 and has been tested in Immunocytochemistry and Western Blotting.

Anti-H2AK15ub Antibody, clone EDL H2AK15-4 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: HIST1H2AG, HIST1H2AK UniProt Number: P0C0S8
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MABE1119
100 μg  
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      개요

      Replacement Information

      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, Dr, HWB, ICCMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABE1119
      DescriptionAnti-H2AK15ub Antibody, clone EDL H2AK15-4
      Alternate Names
      • Histone H2A
      • H2A
      • Histone H2A1
      Background InformationHistone H2A is one of four components of the core nucleosomal structure. Histones undergo a number of post-translational modifications (PTM) in response to various stimuli that may induce changes in the structure of the nucleosome and hide or expose DNA sequences. The degree of ubiquitylation at DNA damage sites is regulated at multiple levels in order to generate proper DNA damage response. Uncontrolled ubiquitylation of histones can lead to unscheduled transcriptional silencing and generate defects in DNA damage checkpoint activation and cell cycle arrest. The RNF168 E3 ligase ubiquitylates H2A at K13, K15, which in turn triggers the recruitment of DNA damage response proteins, such as 53BP1 and BRCA1 to DNA break sites. USP51, a deubiquitinating enzyme, removes H2AK13, 15ub following DNA repair. Depletion of USP51 can result in elevated levels of H2AK13, 15ub on chromatin and cause delays in disassembly of proteins at DNA damage foci.
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2b in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-H2AK15ub, clone EDL H2AK15-4, Cat. No. MABE1119, is a highly specific mouse monoclonal antibody, that targets H2A ubiquitylated at Lys15 and has been tested in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: A representative lot detected H2AK15ub in U2OS cells with or without RNF168 depletion (Wang, Z., et. al. (2016). Genes Dev. 30(8):946-59).


      Immunocytochemistry Analysis: A representative lot detected H2AK15ub in U2OS cells (Wang, Z., et. al. (2016). Genes Dev. 30(8):946-59).
      Biological Information
      ImmunogenKLH-conjugated branched peptide corresponding to the ubiquitination site on human H2AK15.
      CloneEDL H2AK15-4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone EDL H2AK15-4 specifically recognizes histone H2A monoubiquitylated at Lys15, but not unmodified H2A. Does not recognize di-ubiquitin, K63 and K27 linked di-ubiquitin species.
      IsotypeIgG2bκ
      Species Reactivity
      • Mouse
      • Drosophila
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • HIST1H2AG
      • HIST1H2AK
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~25 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Ub-Lysine15 H2A and H2AK13, 15R nucleosome lysates.


      Western Blotting Analysis: 2 ug/mL of this antibody detected Ub-Lysine15 H2A and H2AK13, 15R nucleosome lysates.

      2 mg/mL
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABE1119 04054839168642

      Documentation

      Anti-H2AK15ub Antibody, clone EDL H2AK15-4 MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-H2AK15ub Antibody, clone EDL H2AK15-4 Certificates of Analysis

      TitleLot Number
      Anti-H2AK15ub, clone EDL H2AK15-4 - 3713349 3713349
      Anti-H2AK15ub, clone EDL H2AK15-4 - 3897345 3897345
      Anti-H2AK15ub, clone EDL H2AK15-4 - 4132468 4132468
      Anti-H2AK15ub, clone EDL H2AK15-4 -Q2817663 Q2817663
      Anti-H2AK15ub, clone EDL H2AK15-4 Monoclonal Antibody 3098823
      Anti-H2AK15ub, clone EDL H2AK15-4 Monoclonal Antibody 2968689
      Anti-H2AK15ub, clone EDL H2AK15-4 Monoclonal Antibody 3008130