Millipore Sigma Vibrant Logo

GR01 Anti-EGFR (Ab-1) Mouse mAb (528)

Anti-EGFR (Ab-1), mouse monoclonal, clone 528, recognizes EGF receptor protein in A431 cells and normal skin. It is validated for use in ELISA, IF, IP, IHC (frozen,paraffin) & neutralization studies.

Anti-EGFR (Ab-1) Mouse mAb (528) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Epidermal Growth Factor Receptor

View Products on Sigmaaldrich.com
GR01
가격 및 재고 조회

개요

Replacement Information

주요 사양표

Species ReactivityHostAntibody Type
HMMonoclonal Antibody

가격 및 재고여부

카탈로그 번호 재고 정보패킹 포장 단위 가격(VAT 별도) 수량
GR01-100UGCN
재고 정보 검색...
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      		 Glass bottle 100 μg
      가격 검색...
      가격을 검색할 수 없습니다
      Minimum Quantity needs to be mulitiple of
      Maximum Quantity is
      가격 문의 추가 정보
      ()이 할인됨
       
      —
      가격 문의
      		 —
      Description
      OverviewRecognizes the ~170 kDa EGF receptor protein in A431 cells and normal skin.
      Catalogue NumberGR01
      Brand Family Calbiochem®
      SynonymsAnti-Epidermal Growth Factor Receptor
      References
      ReferencesHunter T. 1984. Nature 311, 414.
      Masui, H., et al. 1984. Cancer Res. 44, 1002.
      Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Product Information
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Negative controlHL-60 cells or human brain
      Positive controlA-431 or normal skin tissue
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Application ReferencesOriginal Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.
      Key Applications Flow Cytometry
      Frozen Sections
      Immunofluorescence
      Immunoprecipitation
      Neutralization Studies
      Paraffin Sections
      Application NotesFlow Cytometry (5 µg/ml)
      Frozen Sections (2.5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Immunoprecipitation(1 µg/sample)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (see application references)
      Application CommentsThis antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogenpartially purified, human EGFR from A431 cells
      ImmunogenHuman
      Epitopewithin the extracellular domain
      Clone528
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      GR01-100UGCN 04055977220162

      Documentation

      Anti-EGFR (Ab-1) Mouse mAb (528) MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-EGFR (Ab-1) Mouse mAb (528) Certificates of Analysis

      TitleLot Number
      GR01

      References

      참고문헌 보기
      Hunter T. 1984. Nature 311, 414.
      Masui, H., et al. 1984. Cancer Res. 44, 1002.
      Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision25-October-2007 JSW
      SynonymsAnti-Epidermal Growth Factor Receptor
      ApplicationFlow Cytometry (5 µg/ml)
      Frozen Sections (2.5 µg/ml)
      Immunofluorescence (5 µg/ml)
      Immunoprecipitation(1 µg/sample)
      Neutralization Studies (see comments and application references)
      Paraffin Sections (see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing BALB/c mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references. Recognizes the ~170 kDa EGF receptor.
      BackgroundEpidermal growth factor (EGF) initiates its effects on cell growth through interaction with a 170,000 dalton cell surface glycoprotein receptor. Binding of EGF or transforming growth factor a to the receptor activates a tyrosine-specific protein kinase intrinsic to the EGF-receptor protein. The phosphotyrosine content of target cells increases rapidly and the EGF-receptor is phosphorylated on tyrosine residues.
      HostMouse
      Immunogen speciesHuman
      Immunogenpartially purified, human EGFR from A431 cells
      Epitopewithin the extracellular domain
      Clone528
      IsotypeIgG2a
      Specieshuman, not mouse, not rat
      Positive controlA-431 or normal skin tissue
      Negative controlHL-60 cells or human brain
      FormLiquid
      FormulationIn 50 mM sodium phosphate buffer, 0.2% gelatin, pH 7.5.
      Concentration Label Please refer to vial label for lot-specific concentration
      Preservative≤0.1% sodium azide
      CommentsThis antibody inhibits EGF binding to its receptor and is an antagonist of in vivo EGF-stimulated tyrosine kinase activity (see application references). Also inhibits A431 tumor formation in nude mice. For neutralization studies, use Cat. No. GR01L; Cat. No. GR01 contains azide, which is toxic to cells. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Yes
      Toxicity Standard Handling
      ReferencesHunter T. 1984. Nature 311, 414.
      Masui, H., et al. 1984. Cancer Res. 44, 1002.
      Reynolds, F. H., Jr., et al. 1981. Nature 292, 259.
      Ushiro, H. and Cohen, S. 1980. J. Biol. Chem. 255, 8363.
      Application referencesOriginal Clone Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Neutralization Studies Gill, G., et al. 1984. J. Biol. Chem. 259, 7761. Kawamoto, T., et al. 1984. J. Biol Chem. 259, 7761. Kawamoto, T., et al. 1983. Prod. Natl. Acad. Sci. USA 80, 1337. Sato, J.D., et al. 1983. Molec. Biol. Med. 1, 511. Paraffin Sections Burton B.C., et al. 2002. Clin. Cancer Res. 1, 180. Mishima, K., et al. 2001. Cancer Res. 61, 7703. Pilcher B.K., et al. 1999. J. Biol. Chem. 274, 10372.

      관련 제품 및 어플리케이션

      Related Products

      카탈로그 번호 설명  
      GR01L Anti-EGFR (Ab-1) Mouse mAb (528) 가격 및 재고 조회

      카테고리

      Life Science Research > Antibodies and Assays > Primary Antibodies