Anti-Cortactin Antibody, phospho-specific [Tyr466]

Cortactin is cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.

Phosphorylated cortactin (Tyr466) synthetic peptide corresponding to mouse amino acid residues around tyrosine 466 (tyrosine 470 in the human sequence).

Research Category
Cell Structure

Research Sub Category
Cytoskeleton

This Anti-Cortactin Antibody, phospho-specific [Tyr466] is validated for use in WB for the detection of Cortactin.

The antibody detects the 80-85 kDa protein of cortactin.

Antibody is cross-adsorbed to nonphospho-peptide corresponding to the site of phosphorylation and preadsorbed with phosphopeptides to cortactin tyrosine 421 and 482 to remove cross-reactivity then affinity purified using phosphorylated cortactin (Tyr466) peptide. Antibody is supplied in phosphate-buffered saline (no Ca²⁺, no Mg²⁺), pH 7.3, containing 1 mg/ml BSA, and 0.05% sodium azide.

Maintain at -20°C for up to one year from date of receipt. Avoid repeated freeze/thaw cycles.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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