Millipore Sigma Vibrant Logo

MABF549 Anti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5

This rat monoclonal antibody Anti-CD25 (mouse), PE-Cy7, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Inflammation & Immunology Gene Symbol: Il2ra, Il2r UniProt Number: P01590
View Products on Sigmaaldrich.com
MABF549
25 µg  
가격 검색...
가격을 검색할 수 없습니다
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
가격 문의 추가 정보
()이 할인됨
 
—
가격 문의
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      특별 제공

       

      고객 서비스로 문의

      개요

      Replacement Information

      특별 제공

      100% Performance Guaranteed

      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MIP, IHC, FCRPE-Cy7Monoclonal Antibody
      Description
      Catalogue NumberMABF549
      DescriptionAnti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5
      Alternate Names
      • Interleukin-2 receptor subunit alpha
      • IL-2 receptor subunit alpha
      • IL-2-RA
      • IL-2R subunit alpha
      • IL2-RA
      • p55
      • CD25
      Background InformationThe PC61.5 antibody is specific for mouse CD25, a 55 kDa surface protein also known as the Interleukin-2 Receptor alpha chain, or IL-2R alpha. CD25 may bind IL-2 by itself, althoµgh with low affinity and without induction of cell signaling. CD25 is also expressed within a high-affinity complex, along with the IL-2R beta chain (CD122) and the common gamma chain (CD132), to form a signaling receptor complex. Expression of CD25 varies during developmental stages of T and B cells, is induced on activated mature T and B cells, and is present on subsets of dendritic cells. CD25 signaling as part of the IL-2 receptor complex triggers T cell activation and proliferation, as well as modulating the differentiation and function of Th17 cells, T regulatory (Treg) cells, and dendritic cells.The PC61.5 antibody is used as a marker for T cells, B cells and dendritic cell subsets. Expression of CD25, CD4 and the transcription factor Foxp3 is regarded as a phenotypic signature for Treg cells. As such, this antibody is widely used for depletion of Treg cells in vivo, as well as to distinguish Treg cells from naïve or conventional T cells which are CD25-.
      References
      Product Information
      FormatPE-Cy7
      PresentationPurified Rat Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.1% gelatin, pH 7.2, and up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal antibody Anti-CD25 (mouse), PE-Cy7, clone PC61.5 validated for use in IP, IHC & Flow Cytometry for the detection of CD25.
      Key Applications
      • Immunoprecipitation
      • Immunohistochemistry
      • Flow Cytometry
      Biological Information
      ImmunogenImmunogen information for this antibody is proprietary information.
      ClonePC61.5
      Concentration0.2 mg/mL
      HostRat
      IsotypeIgG1λ
      Species Reactivity
      • Mouse
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • Il2ra
      • Il2r
      Purification MethodSize Exclusion Chromatography
      UniProt Number
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

      Flow Cytometry Analysis: 0.125 µg of this antibody detected CD25 in one million C57Bl/6 splenocytes.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsIt is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.
      Packaging Information
      Material Size25 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABF549 04053252963148

      관련 제품 및 어플리케이션

      Related Products

      카탈로그 번호 설명  
      MABF396 Anti-CD25 Antibody (mouse), APC, clone PC61.5 가격 및 재고 조회
      MABF397 Anti-CD25 Antibody (mouse), PE-Cy7, clone PC61.5 가격 및 재고 조회
      MABF548 Anti-CD25 Antibody (mouse), APC, clone PC61.5 가격 및 재고 조회
      MABF550 Anti-CD25 Antibody (mouse), PerCP-Cy5.5, clone PC61.5 가격 및 재고 조회

      카테고리

      Life Science Research > Antibodies and Assays > Primary Antibodies