다음 MAP메이트™는 통합될 수 없습니다: -다른 분석 완충용액이 필요한 MAP메이트™. -인산 특이성 및 총 MAP메이트™ 조합, 예: 총 GSK3β 및 GSK3β(Ser 9). -PanTyr 및 자리 특이성 MAP메이트™, 예: Phospho-EGF 수용체 및 phospho-STAT1(Tyr701). -단일 표적(Akt, STAT3)를 위한 1개 이상의 1 phospho-MAP메이트™. - GAPDH 및 β-Tubulin은 panTyr를 포함하는 키트 또는 MAP메이트™와 통합될 수 없습니다.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
Catalogue Number
Ordering Description
Qty/Pack
List
이 제품은 즐겨찾기에 저장되었습니다.
종
패널 유형
선택하신 키트
수량
카탈로그 번호
주문 설명
포장 단위
기재 가격
96-Well Plate
수량
카탈로그 번호
주문 설명
포장 단위
기재 가격
다른 시약 추가 (MAP메이트 사용을 위해 완충용액과 검출 키트가 필요함)
수량
카탈로그 번호
주문 설명
포장 단위
기재 가격
48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
공간 절약 옵션 다수의 키트를 구매하시는 고객은 고용량 저장을 위해 키트 포장을 제거하고 비닐백에 담긴 멀티플레스 분석 구성품을 받아 저장 공간을 절약하도록 선택할 수 있습니다.
이 제품은 즐겨찾기에 저장되었습니다.
해당 제품은 고객님의 카트에 추가되었습니다.
이제 다른 키트를 사용자 지정하거나, 사전 혼합된 키트를 선택하거나, 결재하거나 또는 주문 도구를 종료할 수 있습니다.
B-lymphocyte antigen CD20 (UniProt P11836; also known as B-lymphocyte surface antigen B1, Bp35, CD20, Leukocyte surface antigen Leu-16, Membrane-spanning 4-domains subfamily A member 1) is encoded by the MS4A1 (also known as CD20) gene (Gene ID 931) in human. CD20 is a transmembrane, non-glycosylated protein expressed on B-cell precursors and mature B cells, but is lost following differentiation into plasma cells. In resting B cells, CD20 appears in a 33 kDa non-phosphorylated form. After mitogen stimulation, CD20 becomes heavily phosphorylated (35-37 kDa isoforms), and it is a dominant phosphoprotein in activated B cells and B-cell lines. The long N- and C-terminal ends of the protein are located on the cytoplasmic side of the membrane and only a minor portion of the protein is exposed on the cell surface. It is believed that CD20 plays a direct role in mediating B cells proliferation and differentiation by regulating the transmembrane conductive Ca2+ flux.
References
Product Information
Format
AlexaFluor®647
Presentation
Purified mouse antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.
This Anti-CD20 Antibody, clone FMC7, Alexa Fluor® 647 Conjugate is validated for use in Flow Cytometry for the detection of CD20.
Key Applications
Flow Cytometry
Application Notes
The unconjugated antibody (Cat. No. MAB1217) is shown to be suitable also for Flow cytometry and Western blotting applications.
Biological Information
Immunogen
The human B-lymphoblastoid cell line, HRIK.
Epitope
Extracellular domain
Clone
FMC7
Concentration
Please refer to lot specific datasheet.
Host
Mouse
Specificity
Originally reported to stain majority of prolymphocytic leukemia (B-PLL) and hairy-cell leukemia patients derived B cells (Catovsky, D., et al. (1981). Blood. 58(2):406-408.) and later reported to detect a 105 kDa protein species by immunoblotting (Zola, H., et al. (1987). Dis. Markers. 5(4):227-235.), clone FMC7 is now known to target a conformational epitope on the CD20 extracellular region. Clone FMC7 antigenicity is sensitive to levels of membrane cholesterol. Plamsa membrane cholesterol depletion by methyl-β-cyclodextrin treatment results in greatly reduced Ramos surface staining by clone FMC7 (Deans, J.P., and Polyak, M.J. (2008). Blood. 111(4):2492; author reply 2493-4; Polyak, M.J., et al. (2003). Leukemia. 17(7):1384-1389; Serke, S., et al. (2001). Cytometry. 46(2):98-104).
Flow Cytometry Analysis: 1.0 µg of this antibody detected CD20 in human PBMCs.
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
