Anti-CD20 Antibody, clone FMC7, Alexa Fluor 647 Conjugate

30.1 kDa calculated

The human B-lymphoblastoid cell line, HRIK.

Epitope: Extracellular domain

Originally reported to stain majority of prolymphocytic leukemia (B-PLL) and hairy-cell leukemia patients derived B cells (Catovsky, D., et al. (1981). Blood. 58(2):406-408.) and later reported to detect a 105 kDa protein species by immunoblotting (Zola, H., et al. (1987). Dis. Markers. 5(4):227-235.), clone FMC7 is now known to target a conformational epitope on the CD20 extracellular region. Clone FMC7 antigenicity is sensitive to levels of membrane cholesterol. Plamsa membrane cholesterol depletion by methyl-β-cyclodextrin treatment results in greatly reduced Ramos surface staining by clone FMC7 (Deans, J.P., and Polyak, M.J. (2008). Blood. 111(4):2492; author reply 2493-4; Polyak, M.J., et al. (2003). Leukemia. 17(7):1384-1389; Serke, S., et al. (2001). Cytometry. 46(2):98-104).

Purified mouse antibody conjugate in PBS with 15mg/ml BSA and 0.1 % sodium azide.

Stable for 1 year at 2-8°C from date of receipt.

Evaluated by Flow Cytometry in human PBMCs.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD20 in human PBMCs.

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