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MABN2515-25UG Anti-Alpha Dystroglycan Antibody, clone 3D7

MABN2515-25UG
25 μg  
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      개요

      Replacement Information
      Description
      Catalogue NumberMABN2515-25UG
      DescriptionAnti-Alpha Dystroglycan Antibody, clone 3D7
      Alternate Names
      • Alpha-DGP
      • Alpha-DG
      Background InformationDystroglycan (UniProt: Q62165; also known as Dystrophin-associated glycoprotein 1) is encoded by the Dag1 (also known as Dag-1) gene (Gene ID: 13138) in murine species. Dystroglycan is a component of the dystrophin glycoprotein complex that links the extracellular matrix to the intracellular actin cytoskeleton. It is synthesized with a signal peptide (aa 1-27) that is subsequently cleaved off to generate the mature protein that is further autolytically cleaved (between 651-652) to produce alpha-dystroglycan (aa 28-651) and beta-dystroglycan (aa 652-893). Alpha-dystroglycan is an extracellular peripheral glycoprotein that binds to several extracellular matrix and synaptic proteins such as laminin, agrin, neurexin, and pikachurin. It is reported that its O-mannosyl glycosylation is required for its ligand-binding functions. Beta-dystroglycan is a single-pass type 1 transmembrane protein that plays important roles in connecting the extracellular matrix to the cytoskeleton and serves as a cell adhesion receptor in both muscle and non-muscle tissues. In the cell, beta-dystroglycan binds to dystrophin that is linked to actin cytoskeleton. Hence, alpha/beta dystroglycans act as a molecular axis connecting extracellular matrix with the cytoskeleton across the plasma membrane. Defects in O-mannosyl glycan have been linked to various congenital muscular dystrophies caused by aberrant alpha-dystroglycan glycosylation. (Ref.: Sudo, A., et al. (2018). Human Mol. Genetics. 27(7); 7 1174-1185; Kanagawa, M., et al. (2010). J. Biol. Chem. 285(41); 31208-31216).
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Applications
      ApplicationAnti-Alpha Dystroglycan, clone 3D7, Cat. No. MABN2515, is a rat monoclonal antibody that detects Dystroglycan and has been tested for use in Western Blotting.
      Key Applications
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Alpha Dystroglycan in Western Blotting applications (Ohtsuka, Y., et. al. (2015). Sci Rep. 5:8316; Kanagawa, M., et. al. (2010). J Biol Chem. 285(41):31208-16; Kanagawa, M., et. al. (2016). Cell Rep. 14(9):2209-2223; Manya, H., et. al. (2016). J Biol Chem. 291(47):24618-24627; Sudo, A., et. al. (2018). Hum Mol Genet. 27(7):1174-1185).

      Western Blotting Analysis: 1 µg/mL from a representative lot detected Alpha Dystroglycan in mouse skeletal muscle tissue lysates.
      Biological Information
      ImmunogenMurine alpha-dystroglycan-Fc-Fusion protein.
      Clone3D7
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 3D7 is a rat monoclonal antibody that detects human and murine alpha-dystroglycan. It targets an epitope within the C-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteMouse, Human.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • Dag1
      • Dag-1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~ 97 kDa observed; 96.91 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in mouse brain tissue lysates.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Alpha Dystroglycan in mouse brain tissue lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size25 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      MABN2515-25UG 04054839007071

      Documentation

      Anti-Alpha Dystroglycan Antibody, clone 3D7 MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Anti-Alpha Dystroglycan Antibody, clone 3D7 Certificates of Analysis

      TitleLot Number
      Anti-Alpha Dystroglycan, clone 3D7 - 3245206 3245206

      References

      Reference overviewPub Med ID
      Temporal requirement of dystroglycan glycosylation during brain development and rescue of severe cortical dysplasia via gene delivery in the fetal stage.
      Sudo, A; Kanagawa, M; Kondo, M; Ito, C; Kobayashi, K; Endo, M; Minami, Y; Aiba, A; Toda, T
      Hum Mol Genet  27  1174-1185  2018

      요약 표시
      29360985 29360985
      The Muscular Dystrophy Gene TMEM5 Encodes a Ribitol β1,4-Xylosyltransferase Required for the Functional Glycosylation of Dystroglycan.
      Manya, H; Yamaguchi, Y; Kanagawa, M; Kobayashi, K; Tajiri, M; Akasaka-Manya, K; Kawakami, H; Mizuno, M; Wada, Y; Toda, T; Endo, T
      J Biol Chem  291  24618-24627  2016

      요약 표시
      27733679 27733679
      Identification of a Post-translational Modification with Ribitol-Phosphate and Its Defect in Muscular Dystrophy.
      Kanagawa, M; Kobayashi, K; Tajiri, M; Manya, H; Kuga, A; Yamaguchi, Y; Akasaka-Manya, K; Furukawa, JI; Mizuno, M; Kawakami, H; Shinohara, Y; Wada, Y; Endo, T; Toda, T
      Cell Rep  14  2209-2223  2016

      요약 표시
      26923585 26923585
      Fukutin is prerequisite to ameliorate muscular dystrophic phenotype by myofiber-selective LARGE expression.
      Ohtsuka, Y; Kanagawa, M; Yu, CC; Ito, C; Chiyo, T; Kobayashi, K; Okada, T; Takeda, S; Toda, T
      Sci Rep  5  8316  2015

      요약 표시
      25661440 25661440
      Post-translational maturation of dystroglycan is necessary for pikachurin binding and ribbon synaptic localization.
      Kanagawa, M; Omori, Y; Sato, S; Kobayashi, K; Miyagoe-Suzuki, Y; Takeda, S; Endo, T; Furukawa, T; Toda, T
      J Biol Chem  285  31208-16  2010

      요약 표시
      20682766 20682766