Millipore Sigma Vibrant Logo

ABT1485-AF647 Anti-Actin Antibody, Alexa Fluor® 647 Conjugate

This rabbit polyclonal Anti-Actin, Alexa Fluor® 647 Conjugate, Cat. No. ABT1485-AF647 s validated for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence applications for the detection of Actin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Gene Symbol: ACTA1, ACTA, CFTD, NEM3, MPFD UniProt Number: P68133
View Products on Sigmaaldrich.com
ABT1485-AF647
100 µL  
가격 검색...
가격을 검색할 수 없습니다
Minimum Quantity needs to be mulitiple of
Maximum Quantity is
가격 문의 추가 정보
()이 할인됨
 
—
가격 문의
—
현재 재고 없음 현재 재고 없음
예상 출고 가능일 
단종품
제한된 수량 가능
재고여부 확인
    Remaining: will advise
      Remaining: will advise
      추천사항
      고객 서비스로 문의
      Contact Customer Service

      특별 제공

       

      고객 서비스로 문의

      개요

      Replacement Information

      특별 제공

      100% Performance Guaranteed

      주요 사양표

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, HFC, ICC, IFRbAlexaFluor®647Polyclonal Antibody
      Description
      Catalogue NumberABT1485-AF647
      DescriptionAnti-Actin Antibody, Alexa Fluor® 647 Conjugate
      Alternate Names
      • Actin
      • alpha skeletal muscle
      • Alpha-actin-1
      Background InformationActins are globular multi-functional proteins that serve as the basic building blocks of cytoskeletal microfilaments and are among the most conserved eukaryotic proteins. Six actin types exist, skeletal muscle alpha-actin (ACTA1), smooth muscle alpha-actin (ACTA2), cytoplasmic beta-actin (ACTB), cardiac muscle alpha-actin (ACTC1), cytoplasmic gamma-actin (ACTG1), and smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3). Although actins show more than 90% overall sequence homology, isotypes do show spatial, temporal, and tissue-specific expresson patterns and only 50-60% homology is found in their 18 N-terminal residues. Cytoplasmic β and γ-actins are thought to be present in all cells, while the other four actin isoforms are typically found in specific adult muscle tissue types. Actins exist in a variety of structural states, depending on the specific ionic conditions or the interaction with ligand proteins. The oligomeric and polymeric forms that actin molecules assume are dependent on the distinct conformations they adopt. In additon to serving as the basic building blocks of cytoskeletal microfilaments, actins are involved in multiple nuclear functions and adopt specific conformations that are important for their association with nuclear actin-binding proteins.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified rabbit polyclonal antibody conjugate in PBS with 1.5% BSA and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rabbit polyclonal Anti-Actin, Alexa Fluor® 647 Conjugate, Cat. No. ABT1485-AF647 s validated for use in Flow Cytometry, Immunocytochemistry and Immunofluorescence applications for the detection of Actin.
      Key Applications
      • Flow Cytometry
      • Immunocytochemistry
      • Immunofluorescence
      Application NotesFlow Cytometry Analysis: 2 µL from a representative lot detected actin in one million HeLa cells.

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in NIH/3T3 cells.

      Immunofluorescence Analysis: A 1:50-150 dilution from a representative lot detected actin in human heart, prostate, skeletal muscle, and small intestine tissue sections.

      The unconjugated antibody (Cat. No. ABT1485) and Alexa Fluor® 488-conjugated antibody (Cat. No. ABT1485-AF488) are also available for flow cytometry, immunocytochemistry, immunofluorescence, immunohistochemistry and Western blotting applications.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to the N-terminal end sequence of mature skeletal muscle alpha-actin (ACTA1).
      EpitopeN-terminus
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis Alexa Fluor® 647 conjugate was prepared from purified rabbit polyclonal antibody (Cat. No. ABT1485) raised against skeletal muscle alpha-actin (ACTA1) N-terminal end sequence that shares 80% sequence identity in the corresponding region with cardiac muscle alpha-actin (ACTC1), smooth muscle alpha-actin (ACTA2), and cytoplasmic gamma-actin (ACTG1). Immunogen sequence is not found in smooth muscle gamma-actin (ACTG2, a.k.a. ACTA3) and cytoplasmic beta-actin (ACTB).
      Species Reactivity
      • Mouse
      • Human
      Species Reactivity NoteHuman, Mouse. Broad species reactivity expected due to 100% sequence homology, including Bovine, Horse, Non-Human Primate, Porcine, Rabbit, and Rat.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • ACTA1
      • ACTA
      • CFTD
      • NEM3
      • MPFD
      Purification MethodAffinity purified.
      UniProt Number
      Molecular Weight~42 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected both cytoplasmic and nuclear actin immunoreactivity in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      ABT1485-AF647 04054839083648