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14-279 Akt1/PKBα Protein, inactive, 50 µg

inactive, N-terminal His6-tagged recombinant full-length human Akt1, for use in Kinase Assays.

Akt1/PKBα Protein, inactive, 50 µg MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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14-279
50 µg  
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      개요

      Replacement Information
      Description
      Catalogue Number14-279
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAkt1/PKBα Protein, inactive, 50 µg
      OverviewN-terminal His6-tagged recombinant full-length human Akt1
      References
      Product Information
      Quality LevelMQ100
      Applications
      Applicationinactive, N-terminal His6-tagged recombinant full-length human Akt1, for use in Kinase Assays.
      Key Applications
      • Kinase Assay
      Biological Information
      Sourceexpressed by baculovirus in Sf21 insect cells
      Specific ActivityFor Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
      Entrez Gene Number
      Entrez Gene SummaryThe serine-threonine protein kinase encoded by the AKT1 gene is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. In the developing nervous system AKT is a critical mediator of growth factor-induced neuronal survival. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Multiple alternatively spliced transcript variants have been found for this gene.
      Gene Symbol
      • AKT1
      • RAC
      • PRKBA
      • MGC99656
      • RAC-ALPHA
      • RAC-PK-alpha
      • C-AKT
      • PKB
      • AKT
      Protein TargetAkt1/PKBα
      Purification MethodNi2+/NTA-agarose
      Target Sub-FamilyAGC
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P31749 # General protein kinase capable of phosphorylating several known proteins. Phosphorylates TBC1D4. Signals downstream of phosphatidylinositol 3-kinase (PI(3)K) to mediate the effects of various growth factors such as platelet-derived growth factor (PDGF), epidermal growth factor (EGF), insulin and insulin-like growth factor I (IGF-I). Plays a role in glucose transport by mediating insulin-induced translocation of the GLUT4 glucose transporter to the cell surface. Mediates the antiapoptotic effects of IGF-I. Mediates insulin-stimulated protein synthesis, partly by playing a role in both insulin-induced phosphorylation of 4E-BP1 and in insulin-induced activation of p70 S6 kinase. Promotes glycogen synthesis by mediating the insulin-induced activation of glycogen synthase.
      SIZE: 480 amino acids; 55686 Da
      SUBUNIT: Interacts with CENTG1 isoform 2 (PIKE-A) in the presence of guanine nucleotides. The C-terminus interacts with CCDC88A/GRDN. Interacts with AKTIP.
      SUBCELLULAR LOCATION: Cytoplasm. Nucleus. Note=Nucleus after activation by integrin-linked protein kinase 1 (ILK1).
      TISSUE SPECIFICITY: In all human cell types so far analyzed.
      DOMAIN: SwissProt: P31749 Binding of the PH domain to the phosphatidylinositol 3- kinase alpha (PI(3)K) results in its targeting to the plasma membrane.
      PTM: Phosphorylation on Thr-308, Ser-473 and Tyr-474 is required for full activity. Ser-473 phosphorylation by the Rictor-mTor complex favors Thr-308 phosphorylation by PDPK1. Ser-473 phosphorylation is enhanced by interaction with CENTG1 isoform 2 (PIKE-A).
      SIMILARITY: Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family. RAC subfamily. & Contains 1 AGC-kinase C-terminal domain. & Contains 1 PH domain. & Contains 1 protein kinase domain.
      Molecular Weight59kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assuranceroutinely evaluated by phosphorylation of Crosstide
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage Conditions6 months at -20°C
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      카탈로그 번호 GTIN
      14-279 04053252624872

      Documentation

      Akt1/PKBα Protein, inactive, 50 µg MSDS

      타이틀

      물질안전보건자료(MSDS) 

      Akt1/PKBα Protein, inactive, 50 µg Certificates of Analysis

      TitleLot Number
      Akt1/PKB#945;, unactive - D8SN004U-N D8SN004U-N
      Akt1/PKB#945;, unactive - D8SN004U-O D8SN004U-O
      Akt1/PKB#945;, unactive - D8SN004U-P D8SN004U-P
      Akt1/PKB#945;, unactive - D8SN004U-Q D8SN004U-Q
      Akt1/PKB#945;, unactive - D8SN004U-R D8SN004U-R
      Akt1/PKB#945;, unactive - D8SN004U-S D8SN004U-S
      Akt1/PKB#945;, unactive - D8SN004U-T D8SN004U-T
      Akt1/PKBα, unactive - D8SN004U-U D8SN004U-U

      References

      Reference overviewApplicationPub Med ID
      Stoichiometry of site-specific protein phosphorylation estimated with phosphopeptide-specific antibodies.
      Mîinea, Cristinel P and Lienhard, Gustav E
      BioTechniques, 34: 828-31 (2003)  2003

      		Kinase Assay12703308 12703308
      Synergistic Activation of Endothelial Nitric-oxide Synthase (eNOS) by HSP90 and Akt: CALCIUM-INDEPENDENT eNOS ACTIVATION INVOLVES FORMATION OF AN HSP90-Akt-CaM-BOUND eNOS COMPLEX
      Takahashi, S. and Mendelsohn, M. E.
      J Biol Chem, 278:30821-7 (2003)  2003

      		Kinase Assay12799359 12799359
      Inhibition of integrin-linked kinase/protein kinase B/Akt signaling: mechanism for ganglioside-induced apoptosis
      Wang, X. Q., et al
      J Biol Chem, 276:44504-11 (2001)  2001

      		Immunoprecipitation, Immunoblotting (Western)11577096 11577096
      Complex formation and cooperation of protein kinase C theta and Akt1/protein kinase B alpha in the NF-kappa B transactivation cascade in Jurkat T cells
      Bauer, B., et al
      J Biol Chem, 276:31627-34 (2001)  2001

      		Kinase Assay11410591 11410591
      3-Phosphoinositide-dependent protein kinase 1 (PDK1) phosphorylates and activates the p70 S6 kinase in vivo and in vitro.
      Alessi, D R, et al.
      Curr. Biol., 8: 69-81 (1998)  1998

      		9427642 9427642
      Characterization of a 3-phosphoinositide-dependent protein kinase which phosphorylates and activates protein kinase Balpha.
      Alessi, D R, et al.
      Curr. Biol., 7: 261-9 (1997)  1997

      		9094314 9094314
      Mechanism of activation of protein kinase B by insulin and IGF-1.
      Alessi, D R, et al.
      EMBO J., 15: 6541-51 (1996)  1996

      요약 표시       		8978681 8978681