Highest Purity Silica

Purospher® HPLC Columns: Enhanced Performance for Best Results

Purospher® HPLC columns are based on high-purity, metal-free silica to ensure excellent separations with symmetrical peak shape. The silica is manufactured in a sol-gel process from tetraalkoxysilane, which results in a purity of 99.999%, and metal content of less than 5 ppm (parts per million). Hence, Purospher® columns are the optimal choice for outstanding, tailing-free separations and enhanced performance.

Metal Contaminants in Base Silica

Since Purospher® columns are produced using ultra-high purity silica with virtually no metal contaminants, no chelate complexes are formed during chromatography.

Purospher® Si HPLC Columns

The unique purity of Purospher® Si is clearly shown in the mobile phase system n-hepatane/dioxane. The highly sensitive metal-complexing agents, Quinizarin (1) and 2,2'-Bipyridine (2), are symmetrically eluted.

See details

Mobile Phase	n-Heptane/Dioxane (90/10, v/v)
Flow rate	1.0 mL/min
Detection	UV 254 nm
Temperature	30° C

Purospher® STAR RP-18 Endcapped HPLC Columns

There are differences in the quality of so-called "high purity" HPLC column sorbents. The peak shape of the strong metal chelator Quinizarin is an ideal indicator of silica purity. Compared to any column in its class, Purospher® STAR RP-18 endcapped achieves the best peak shape and symmetry factor value for Quinizarin, This is thanks to its extremely low metal content of less than 5 ppm.

See details

Mobile Phase	Methanol / Buffer pH 7; 80 / 20 (5 mmol KH2PO4 and 5 mmol K2HPO4 )
Flow rate	1.0 mL/min
Detection	UV 254 nm
Temperature	22°C
Sample	1. Uracil 2. Toluene 3. Ethylbenzene 4. Quinizarin 5. Amitriptyline