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565834 sFRP-3, His•Tag®, Mouse, Recombinant

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565834
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Overview

Replacement Information

Key Spec Table

Purity
≥95% by SDS-PAGE

Pricing & Availability

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565834-10UGCN
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      Description
      OverviewRecombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
      Catalogue Number565834
      Brand Family Calbiochem®
      References
      ReferencesHoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.
      Product Information
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Quality LevelMQ100
      Applications
      Biological Information
      Biological activityProliferation Assay:
      ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.

      Functional ELISA:
      50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
      Purity≥95% by SDS-PAGE
      Physicochemical Information
      ContaminantsEndotxin: ≤1 EU/µg
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      565834-10UGCN 04055977191912

      Documentation

      sFRP-3, His•Tag®, Mouse, Recombinant SDS

      Title

      Safety Data Sheet (SDS) 

      sFRP-3, His•Tag®, Mouse, Recombinant Certificates of Analysis

      TitleLot Number
      565834

      References

      Reference overview
      Hoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision21-Sepmtember-2011 RFH
      DescriptionRecombinant, mouse secreted frizzled related protein 3 (sFRP-3) consisting of amino acids Ala 33-Ser323, fused at the N-terminus to a His•Tag® sequence and expressed in NSO-derived mouse myeloma cells. sFRP-3 is a soluble antagonist of Wnt signaling. It prohibits interactions with Frizzled receptors by binding directly to Wnt proteins.
      FormLyophilized
      FormulationLyophilized from 0.2 µm filtered PBS, BSA.
      Purity≥95% by SDS-PAGE
      ContaminantsEndotxin: ≤1 EU/µg
      Biological activityProliferation Assay:
      ED₅₀ = 1.6-6 µg/ml as measured by inhibition of proliferation of MC3T3-E1 mouse osteoblastic cells.

      Functional ELISA:
      50% optimal binding response = 150-250 ng/ml as measured by binding ro rmWnt-3a (500 ng/ml).
      SolubilityReconstitute with sterile PBS contanining ≥1% human or bovine serum albumin. Please refer to the vial label for lot-specific reconstitution volume.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstituion refrigerate (4°C) for short term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -20°C.
      Toxicity Standard Handling
      ReferencesHoang, et al. 1996. J. Biol. Chem. 271, 26131. Leyns, et al. 1997 Cell 88, 747. Wang, et al. 1997 Cell 88, 757. Mayr, et al. 1997 Mech. Dev. 63, 109. Rattner, et al. 1997. PNAS 94, 2859.