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Merck

ISEQ07850

Immobilon® -PSQ PVDF Membrane

50 sheets, 7 cm x 8.4 cm, 0.2 µm pore size, transfer membrane for Western blotting

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About This Item

UNSPSC Code:
41105339
eCl@ss:
32031602
NACRES:
NB.22
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Product Name

Immobilon®-PSQ PVDF Membrane, 50 sheets, 7 cm x 8.4 cm, 0.2 µm pore size, Hydrophobic PVDF Transfer Membrane for western blotting.

material

PVDF membrane
plain filter
white filter

feature

hydrophobic

manufacturer/tradename

Immobilon®

technique(s)

dot blot: suitable
western blot: suitable

filter L × W

7 cm × 8.4 cm

pore size

0.2 μm pore size

capacity

262 μg/cm2 adsorption capacity (insulin)
340 μg/cm2 adsorption capacity (BSA)
448 μg/cm2 adsorption capacity (goat IgG)

compatibility

for use with Amido black
for use with CPTS
for use with Colloidal gold
for use with Coomassie brilliant blue
for use with India ink
for use with Ponceau-S red
for use with Toluidine blue
for use with Transillumination

detection method

chemiluminescent
colorimetric
fluorometric
radioactive

shipped in

ambient

Quality Level

Application

Immobilon-PSQ PVDF Membrane has been used for western blotting.

Features and Benefits

Immobilon-PSQ Membrane:
  • Compatible with tank and semi-dry electroblotting systems
  • Higher protein adsorption and sequencing yields than other membranes
  • Prevents blow-through of low molecular weight proteins

General description

The Immobilon®-PSQ membrane is a 0.2 μm microporous polyvinylidene fluoride (PVDF) membrane that has been developed to maximize protein binding. This transfer membrane is suitable for direct protein sequencing and immunodetection following electroblotting of protein from electrophoresis gels (or dot binding of purified protein samples). The Immobilon®-PSQ membrane offers optimal blotting for proteins with molecular weights less than 20 kDa. It has more surface area and displays higher binding capacity for small proteins.
Filter Code: ISEQ
Primary Binding Mechanisms: Electrostatic, hydrophobic

Legal Information

Immobilon is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10-13 - German Storage Class 10 to 13


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Life science alliance, 3(1) (2019-12-11)
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Protocols

폴리아크릴아미드 겔 화학의 개요와 mPAGE® TurboMix Bis-Tris 겔 캐스팅 키트를 이용한 폴리아크릴아미드 겔 핸드캐스팅에 대한 상세 지침

Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.

Related Content

There’s so much room for experimental variability in traditional immunodetection workflows. For your peace of mind – and ours – we designed the SNAP i.d.® 2.0 system to streamline your Western blot and immunohistochemistry experiments. The concept is simple: a vacuum-driven flow of blocking, antibody, and washing solutions reduces slide and membrane handling. That means a lot less shaking, dipping, pouring and waiting. And now you can process multiple blots and slides in parallel, so it’s easy to apply consistent conditions across experiments.

Antibody reuse for Western blotting is a common practice for many researchers. While many antibodies lose potency with time or degrade even faster due to improper storage conditions, it is important to recognize the potential value of recovering the primary antibody for possible reuse in some experiments. The SNAP i.d.® 2.0 system is not only able to reduce the immunodetection processing time, but its flexibility lets you combine conditions used in the standard immunodetection protocol and also allows the collection of antibody for future reuse. Here, we compare the antibody recovery and reuse in the standard immunodetection protocol with the antibody recovery and reuse in SNAP i.d.® system using the extended protocol and the original SNAP i.d.® protocol.

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Global Trade Item Number

SKUGTIN
ISEQ0785004053252269950

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