Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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654245
Sigma-AldrichTNF-α, Mouse, Recombinant, E. coli
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Description
Overview
Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. Referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune systems response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
Catalogue Number
654245
Brand Family
Calbiochem®
Synonyms
rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
References
References
Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293. Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
Product Information
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Purity
≥97% by SDS-PAGE
Physicochemical Information
Contaminants
Endotoxin: ≤100 pg/µg TNF-α
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
≤ -70°C
Do not freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
TNF-α, Mouse, Recombinant, E. coli Certificates of Analysis
Title
Lot Number
654245
References
Reference overview
Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293. Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
Data Sheet
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Revision
19-May-2008 RFH
Synonyms
rmTNF-α, rm-Cachectin, Tumor Necrosis Factor-α
Description
Recombinant, mouse tumor necrosis factor-α expressed in E. coli. Has the ability to kill certain tumor cells directly. It is referred to as an inflammatory cytokine as it initiates the cascade of other cytokines and factors that make up the immune system's response to infection and cancer. Reported to mediate changes in bone metabolism during inflammation.
Form
Lyophilized
Formulation
Lyophilized from sterile-filtered PBS, 50 µg BSA/µg TNF-α.
Purity
≥97% by SDS-PAGE
Contaminants
Endotoxin: ≤100 pg/µg TNF-α
Biological activity
ED₅₀ = 20-50 pg/ml as measured in a cytotoxic assay using the TNF-α susceptible murine L-929 cell line in the presence of Actinomycin D (Cat. No. 114666)
Solubility
Reconstitute to a concentration ≥10 µg/ml with sterile PBS containing ≥0.1% HSA or BSA.
Storage
≤ -70°C
Do Not Freeze
Ok to freeze
Special Instructions
Following reconstitution, aliquot and freeze (-70°C) for long term storage or refrigerate (4°C) for short term storage. Avoid freeze/thaw cycles of solutions. Stock solutions are stable for up to 1 month at 4°C or for up to 3 months at -70°C.
Toxicity
Standard Handling
Merck USA index
14, 9812
References
Scharla, S.H., et al. 1994. Eur. J. Endocrinol. 131, 293. Eck, M.J., et al. 1992. J. Biol. Chem. 267, 2119.
