Millipore Sigma Vibrant Logo

69522 T7•Tag® Monoclonal Antibody

The T7•Tag antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors.

T7•Tag® Monoclonal Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
View Products on Sigmaaldrich.com
69522
View Pricing & Availability

Overview

Replacement Information

Pricing & Availability

Catalogue Number AvailabilityPackaging Qty/Pack Price Quantity
69522-3CN
Retrieving availability...
—
Limited Availability Limited Availability
In Stock 
Discontinued
Limited Quantities Available
Availability to be confirmed
    Remaining : Will advise
      Remaining : Will advise
      Will advise
      Contact Customer Service
      Contact Customer Service

      		 Plastic ampoule 50 μg
      Retrieving price...
      Price could not be retrieved
      Minimum Quantity is a multiple of
      Maximum Quantity is
      Upon Order Completion More Information
      You Saved ()
       
      —
      Request Pricing
      		 —
      69522-4CN
      Retrieving availability...
      —
      Limited Availability Limited Availability
      In Stock 
      Discontinued
      Limited Quantities Available
      Availability to be confirmed
        Remaining : Will advise
          Remaining : Will advise
          Will advise
          Contact Customer Service
          Contact Customer Service

          		 Plastic ampoule 250 μg
          Retrieving price...
          Price could not be retrieved
          Minimum Quantity is a multiple of
          Maximum Quantity is
          Upon Order Completion More Information
          You Saved ()
           
          —
          Request Pricing
          		 —
          Description
          OverviewThe T7•Tag® Antibody is a mouse monoclonal antibody (IgG2b) directed against the 11 amino acid gene 10 leader peptide expressed by many of the pET translation vectors as well as pSCREEN™ and pRSET vectors. Because the peptide is the natural amino terminal end of the T7 major capsid protein, the antibody also recognizes T7 bacteriophage (e.g., the T7Select™ Vectors). For increased versatility and sensitivity, this antibody is also available labeled with biotin and as a direct conjugate with alkaline phosphatase or horseradish peroxidase.
          SpecificityT7•Tag peptide MetAlaSerMetThrGlyGlyGlnGlnMetGly; N-terminal, C-terminal or internal
          Species/IsotypeMouse monoclonal IgG2b
          Cross-reactivityNegligible with bacterial, insect or mammalian cell lysates
          Sensitivity1–10 ng (Western blot developed with chromogenic substrates)
          < 1 ng (AP or HRP conjugate developed with chemiluminescent substrates, or Biotin conjugate developed with Streptavidin AP and chromogenic substrates). Also suitable for immunoprecipitation and immunohistochemistry (1–3)
          FormStabilized solution of antibody in 50% glycerol; includes Positive Control Extract containing 31.1 kDa target protein
          Working dilution1:5,000–1:10,000
          Catalogue Number69522
          Brand Family Novagen®
          References
          References1. Krek, W., et al. 1994. Cell 78,, 161.

          2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

          3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.
          Product Information
          Components
          Quality LevelMQ100
          Applications
          Biological Information
          Physicochemical Information
          Dimensions
          Materials Information
          Toxicological Information
          Safety Information according to GHS
          Safety Information
          Product Usage Statements
          Storage and Shipping Information
          Ship Code Shipped with Blue Ice or with Dry Ice
          Toxicity Multiple Toxicity Values, refer to MSDS
          Storage -20°C
          Avoid freeze/thaw Avoid freeze/thaw
          Do not freeze Ok to freeze
          Packaging Information
          Transport Information
          Supplemental Information
          Specifications
          Global Trade Item Number
          Catalogue Number GTIN
          69522-3CN 04055977274448
          69522-4CN 07790788052584

          Documentation

          T7•Tag® Monoclonal Antibody SDS

          Title

          Safety Data Sheet (SDS) 

          T7•Tag® Monoclonal Antibody Certificates of Analysis

          TitleLot Number
          69522

          References

          Reference overview
          1. Krek, W., et al. 1994. Cell 78,, 161.

          2. Roberts, S.G.E. and Green, M. (1994) Nature 371, 717–720.

          3. Joneson, T., McDonough, M., Bar-Sag, D., and Van Aelst, L. (1996) Science 274, 1374–1376.

          Citations

          Title
        • Clock, S.A., et al. 2008. Journal of Bacteriology 190, 980.
        • Chunying Song, et al. 2008. Journal of Biological Chemistry 283, 4004.
        • Cinar, B., et al. 2007. Journal of Biological Chemistry 282, 29584.
        • Cocuron, J., et al. 2007. Procedings of the National Academy of Science 104, 8550.
        • Furuyama, S. and Biggins S., 2007. Procedings of the National Academy of Science 104, 14706.
        • Geisler, D.A, et al. 2007. Journal of Biological Chemistry 282, 28455.
        • Goodier, J.L., et al. 2007. Molecular and Cellular Biology 27, 6469.
        • Hayakawa, H., et al. 2007. "http://www.jbc.org/content/282/36/26369.abstract">Journal of Biological Chemistry 282, 26369.
        • Kennedy, N.J., et al. 2007. Genes and Development 21, 2336.
        • Maddugoda, M.P., et al. 2007. Journal of Cell Biology 178, 529.
        • Schoonheim, P.J., et al. 2007. Plant Physiology 143, 670.
        • Ye, Z., et al. 2007. American Journal of Pathology 171, 882.
        • Bedet, C., et al. 2006. Journal of Biological Chemistry 281, 30046.
        • Bor, Y., et al. 2006. Genes and Development 20, 1597.
        • Chia, D.J., et al. 2006. Journal of Biological Chemistry 281, 3190.
        • Dance, M., et al. 2006. Journal of Biological Chemistry 281, 23285.
        • Johnson, E.A., et al. 2006. Molecular Pharmacology 70, 676.
        • Leger, A.J., et al. 2006. Circulation 113, 1244.
        • Nowak, J.E., et al. 2006. Bio/Techniques 40, 220.
        • Oh, R.S., et al. 2006. Journal of Biological Chemistry 281, 21480.
        • Rayala, S.K., et al. (2006. Journal of Biological Chemistry 281, 4395.
        • Taira, H., et al. 2006. Nucleic Acids Research 34, 1653.
        • Uchimura, Y., et al. 2006. Journal of Biological Chemistry 281, 23180-23190.
        • Zhang, F., et al. 2006. Journal of Biological Chemistry 281, 30036.
        • Allemand, E., et al. 2005. Proceedings of the National Academy of Sciences (USA) 102, 3605.
        • Bairstow, S.F., et al. 2005. Journal of Biological Chemistry 280, 23884.
        • Barboric, M., et al. 2005. European Molecular Biology Organization Journal 24, 4291.
        • Barnes, C.J., et al. 2005. Clinical Cancer Research 11, 2868.
        • Chisholm, S.T., et al. 2005. Procedings of the National Academy of Science 102, 2087.
        • Chuang C., and Bargmann, C.I., 2005. Genes and Development 19, 270.
        • Cui, H., et al. 2005. Journal of Biological Chemistry 280, 9474.
        • Darnell, J.C., et al. 2005. Genes and Development 19, 903.
        • Dasgupta, B., et al. 2005. Cancer Research 65, 2755.
        • Dasgupta, B. et al. 2005. Cancer Research 65, 236.
        • Dredge, B.K., et al. 2005. European Molecular Biology Organization Journal 24, 1608.
        • Georgescu, S.P., et al. 2005. Molecular Endocrinology 19, 2491.
        • Huang, A., et al. 2005. Cancer Research 65, 5607.
        • Katagiri, C., et al. 2005. Journal of Biological Chemistry 280, 14716.
        • Kotoshiba, S., et al. 2005. Journal of Biological Chemistry 280, 17694.
        • Kray, A.E., et al. 2005. Journal of Biological Chemistry 280, 35974.
        • Kuroda T.S., and Fukuda, M., 2005. Journal of Biological Chemistry 280, 28015.
        • Lin, Y., et al. 2005. Journal of Biological Chemistry 280, 12799.
        • Lucet, I.S., et al. 2005. Journal of Biological Chemistry 280, 2503.
        • Miyake, Y., et al. 2005. Journal of Biological Chemistry 280, 12643.
        • Miyase, S., et al. 2005. Journal of Biological Chemistry 280, 515.
        • Nakamura, N., et al. 2005. Journal of Biological Chemistry 280, 1561.
        • Papandreou, I., et al. 2005. Cancer Research 65, 3171.
        • Qiu, R., et al. 2005. Journal of Biological Chemistry 280, 16695.
        • Robitaille, H., et al. 2005. Journal of Biological Chemistry 280, 12732.
        • Sun, L., et al. 2005. Journal of Biological Chemistry 280, 26483.
        • Suzuki, K., et al. 2005. Biophysical Journal 88, 3659.
        • Thomee, C., et al. 2005. Journal of Clinical Endocrinology and Metabolism 90, 2487.
        • Vo, A.T., et al. 2005. European Molecular Biology Organization Reports 6, 438.
        • Wente, W., et al. 2005. Journal of Biological Chemistry 280, 32419.
        • Wheeler, D.L., et al. 2005. American Journal of Pathology 166, 117.
        • Yanagi, K., et al. 2005. Journal of Biological Chemistry 280, 19689.
        • Zhao X., and Blobel G., 2005. Proceedings of the National Academy of Sciences (USA) 102, 4777.
        • Ball, J.R., et al. 2004. RNA 10, 19.
        • Fukuda, M. and Kuroda T.S., 2004. Journal of Cell Science 117, 583.
        • Kurebayashi, S., et al. 2004. Biochemical and Biophysical Research Communications 315, 919.
        • Tan, W., et al. 2004. Journal of Biological Chemistry 279, 55153.
        • Cobbold, C., et al. 2003. Human Molecular Genetics 12, 1523.
        • Kagey, M.H., et al. 2003. Cell 113, 127.
        • Kim E.J., and Helfman, D.M., 2003. Journal of Biological Chemistry 278, 30063.
        • Han, Z., et al. 2002. Biochemical and Biophysical Research Communications 292, 1036.
        • Zhong, H., et al. 2002. Nature 420, 193.
        • Sekiguchi, T., et al. 2001. Journal of Biological Chemistry 276, 7246.
        • Morimura, T., et al. 2000. Journal of Biological Chemistry 275, 36523.

          • User Protocols

          Title
          TB015 T7•Tag® Antibody

          Related Products & Applications

          Related Products

          Catalogue Number Description  
          69048 T7•Tag® Antibody HRP Conjugate Show Pricing & Availability

          Related Products By: Brand Facete

          T7•Tag®

          Categories

          Life Science Research > Antibodies and Assays > Primary Antibodies