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361527 PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3)

This PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3) is validated for use in ELISA, Immunoblotting for the detection of GSK3β (pSer⁹).

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
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361527
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, MMMonoclonal Antibody

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      Description
      OverviewRecognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
      Catalogue Number361527
      Brand Family Calbiochem®
      References
      Product Information
      FormLiquid
      Formulation100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
      Positive controlPervanadate-treated HepG2 (included)
      Preservative≤0.1% sodium azide (antibody only)
      Quality LevelMQ100
      Applications
      Key Applications Enzyme-Linked Immunosorbent Assay
      Immunoblotting (Western Blotting)
      Application NotesELISA (0.05 µg/ml)
      Immunoblotting (0.5 µg/ml)
      Application CommentsFor immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
      ImmunogenHuman
      Clone2D3
      HostMouse
      IsotypeIgG₁
      Species Reactivity
      • Human
      • Mouse
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      361527-1SETCN 04055977192940

      Documentation

      PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3) SDS

      Title

      Safety Data Sheet (SDS) 

      PhosphoDetect™ Anti-GSK3β (pSer⁹) Mouse mAb (2D3) Certificates of Analysis

      TitleLot Number
      361527
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision14-September-2009 RFH
      ApplicationELISA (0.05 µg/ml)
      Immunoblotting (0.5 µg/ml)
      DescriptionMouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~46 kDa GSK-3β phosphorylated at Ser9. Supplied with a control lysate derived from 2 x 106 EGF-treated HepG2 cells.
      HostMouse
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser⁹ phosphorylation site of human glycogen synthase kinase 3β
      Clone2D3
      IsotypeIgG₁
      Specieshuman, mouse
      Positive controlPervanadate-treated HepG2 (included)
      FormLiquid
      Formulation100 µg antibody in PBS, PEG, sucrose, 50% glycerol and 200 µl lyophilized control lysate.
      Preservative≤0.1% sodium azide (antibody only)
      CommentsFor immunoblotting with the positive control, use 10 µl (chemiluminescence) or 20 µl (colorimetric) per mini-gel lane. Variables associated with assay conditions will dictate the proper working dilution.
      Storage -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the positive control lysate with 200 µl H₂O. Following complete solubilization, add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min.
      Toxicity Standard Handling

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies