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341292 PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb

This PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb is validated for use in Immunoblotting, Immunohistochemistry for the detection of FAK (pTyr³⁹⁷).

PhosphoDetect™ Anti-FAK (pTyr³⁹⁷) Rabbit pAb MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)

341292
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Overview

Key Spec Table

Species ReactivityHostAntibody Type
Ch, Dr, Fg, H, M, RRbPolyclonal Antibody

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      Description
      OverviewRecognizes the ~125 kDa FAK protein when phosphorylated at Tyr397. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
      Catalogue Number341292
      Brand Family Calbiochem®
      SynonymsAnti-Focal Adhesion Kinase, Phospho-Specific (Tyr³⁹⁷)
      Application Data
      Detection of FAK, phosphorylated on Tyr397, by immunoblotting. Sample: Whole cell lysate from CEF cells expressing FAK and plated on fibronectin. Primary antibody: PhosphoDetect™ Anti-FAK (pTyr397) Rabbit pAb (Cat. No. 341292) (1:000) pre-incubated without peptide (lanes 1), non-phosphopeptide corresponding to the immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), phosphopeptide derived from the corresponding region of Pyk2 (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      References
      ReferencesFornaro, M., et al. 2000. Mol. Biol. Cell 11, 2235.
      Taylor, J, et al. 2000. J. Biol. Chem. 275, 19250.
      Vial, D., et al. 2000. J. Biol. Chem. 275, 28269.
      Wennerberg, K., et al. 2000. Mol. Cell. Biol. 20, 5758.
      Ridyard, M.S., and Sanders, E.J. 1999. Anat. Embryol. (Berl.) 199, 1.
      Product Information
      FormLiquid
      FormulationIn Dulbecco's PBS (no Mg²+ and Ca²+), 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Positive controlChicken embryo fibroblasts expressing FAK or NIH3T3 cells treated with PDGF.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunohistochemistry
      Application NotesImmunoblotting (1:1000)
      Immunohistochemistry (see comments; heat pre-treatment required)
      Application CommentsAlthough the antibody was adsorbed against a phospho-peptide corresponding to Pyk2, some cross-reactivity may still be detected in cases where Pyk2 is overexpressed relative to FAK within the same cell system. This antibody has also been reported to work for immunohistochemistry; heat pre-treatment with citrate buffer, pH 6.0 is required. When diluted 1:1000 there is sufficient antibody for 10 miniblots at 10 ml per blot. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Tyr³⁹⁷ phosphorylation site of human FAK
      HostRabbit
      IsotypeIgG
      Species Reactivity
      • Chicken
      • Drosophila
      • Frog
      • Human
      • Mouse
      • Rat
      Antibody TypePolyclonal Antibody
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Global Trade Item Number
      Catalogue Number GTIN
      341292-10TCN 04055977215625