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17-126 PTP Assay Kit 2

PTP Assay Kit 2 can be used to detect PTP-1B activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.

PTP Assay Kit 2 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Phosphatase Assay
17-126
1 kit  
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      Overview

      Replacement Information

      Key Spec Table

      Key ApplicationsAnalytes AvailableDetection Methods
      PAPTP Colorimetric
      Description
      Catalogue Number17-126
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionPTP Assay Kit 2
      References
      Product Information
      Components
      • Tyrosine Phosphopeptide (TSTEPQpYQPGENL) (Cat.# 12-218)
      • Malachite Green Solution A (Cat.# 20-105)
      • Malachite Green Additive (Cat.# 20-104)
      • Phosphate Standard (Cat.# 20-103)
      • pNPP (p-Nitrophenyl Phosphate) (Cat.# 20-106)
      • pNPP Tyr Assay Buffer (Cat.# 20-180)
      • 96-well microtiter plate
      Detection methodColorimetric
      Quality LevelMQ100
      Applications
      ApplicationPTP Assay Kit 2 can be used to detect PTP-1B activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.
      Key Applications
      • Phosphatase Assay
      Biological Information
      Analytes Available
      • PTP
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is the founding member of the protein tyrosine phosphatase (PTP) family, which was isolated and identified based on its enzymatic activity and amino acid sequence. PTPs catalyze the hydrolysis of the phosphate monoesters specifically on tyrosine residues. Members of the PTP family share a highly conserved catalytic motif, which is essential for the catalytic activity. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP has been shown to act as a negative regulator of insulin signaling by dephosphorylating the phosphotryosine residues of insulin receptor kinase. This PTP was also reported to dephosphorylate epidermal growth factor receptor kinase, as well as JAK2 and TYK2 kinases, which implicated the role of this PTP in cell growth control, and cell response to interferon stimulation.
      Gene Symbol
      • PTPN1
      • PTP-1B
      • PTP1B
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P18031 # May play an important role in CKII- and p60c-src-induced signal transduction cascades (By similarity).
      SIZE: 435 amino acids; 49967 Da
      SUBCELLULAR LOCATION: Endoplasmic reticulum membrane; Peripheral membrane protein; Cytoplasmic side.
      PTM: Oxidized on Cys-215; the Cys-SOH formed in response to redox signaling reacts with the alpha-amido of the following residue to form a 4-amino-3-isothiazolidinone serine cross-link, triggering a conformational change that inhibits substrate binding and activity. The active site can be restored by reduction.
      SIMILARITY: SwissProt: P18031 ## Belongs to the protein-tyrosine phosphatase family. Non-receptor class 1 subfamily. & Contains 1 tyrosine-protein phosphatase domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceThe components in this kit were used to detect PTP-1B (Catalog # 14-109) activity by either dephosphorylation of the phosphopeptide (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) or hydrolysis of pNPP.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Packaging Information
      Material Size1 kit
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      17-126 04053252407222

      Documentation

      PTP Assay Kit 2 SDS

      Title

      Safety Data Sheet (SDS) 

      PTP Assay Kit 2 Certificates of Analysis

      TitleLot Number
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2459578 2459578
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 3125647
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2970636
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2927702
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 3030840
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2905533
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2896282
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) 2958930
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2197520 2197520
      PTP Assay Kit 2 (T-S-T-E-P-Q-pY-Q-P-G-E-N-L) - 2279221 2279221
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      References

      Reference overviewApplicationPub Med ID
      The serine phosphatases PP1 and PP2A associate with and activate the actin-binding protein cofilin in human T lymphocytes
      Ambach, A., et al
      Eur J Immunol, 30:3422-31 (2000)  2000

      		Phosphatase Assay11093160 11093160
      Phosphorylated synthetic peptides as tools for studying protein phosphatases
      Pinna, L. A. and Donella-Deana, A.
      Biochim Biophys Acta, 1222:415-31 (1994)  1994

      		Phosphatase Assay8038211 8038211
      Inorganic and organic phosphate measurements in the nanomolar range.
      Van Veldhoven, P P and Mannaerts, G P
      Anal. Biochem., 161: 45-8 (1987)  1987

      Show Abstract       		Phosphatase Assay3578786 3578786

      FAQ

      QuestionAnswer
      I still have material left to run more assays, but I need another plate. Where can I find a comparable plate?To get a comparable plate, you can purchase a Costar Brand 96-Well Half Area Plate (for Tissue Culture Section).

      Related Products & Applications

      Kit Component

      Catalogue Number Description  
      12-218 Tyrosine Phosphopeptide (TSTEPQpYQPGENL) Show Pricing & Availability
      20-103 Phosphate Standard Show Pricing & Availability
      20-104 Malachite Green Additive Show Pricing & Availability
      20-105 Malachite Green Solution A Show Pricing & Availability
      20-106 pNPP (p-Nitrophenyl Phosphate) Show Pricing & Availability
      20-180 pNPP Tyr Assay Buffer Show Pricing & Availability

      Categories

      Life Science Research > Protein Detection and Quantification > Activity Assays > Phosphatase Assays