Millipore Sigma Vibrant Logo

RMNPMAG-83K MILLIPLEX MAP Rat/Mouse Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay

The analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Key Applications: Multiplexing Detection Methods: Luminex xMAP Research Category: Neuroscience
View Products on Sigmaaldrich.com
RMNPMAG-83K
Design And Price Your Kit

Overview

Key Spec Table

Analytes AvailableSpecies ReactivityKey ApplicationsDetection Methods
α-MSH β-Endorphin Neurotensin Oxytocin Substance P R, MMplexLuminex xMAP
Description
Catalogue NumberRMNPMAG-83K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX MAP Rat/Mouse Neuropeptide Magnetic Bead Panel - Neuroscience Multiplex Assay
OverviewThe MILLIPLEX® MAP Rat/Mouse Neuropeptide Magnetic Bead Panel, containing 5 biomarkers. This kit may be used for the analysis of all or any combination of the above analytes in tissue/cell lysate/culture supernatant samples and cerebrospinal fluid (CSF). Serum or plasma samples may also be used, and will need to be extracted, however we do not recommend using the panel for serum and plasma Substance P. The kit contains enough material to analyze 38 samples in duplicate, including the standard curve and two quality controls.

An important note about this magnetic panel is that the samples may be extracted using either the acetonitrile precipitation technique or the Waters 96-well HLB extraction plate. Please refer to the protocol for more information on both methods. The necessary components to perform the extractions are not included with the kit.
Background InformationThe central nervous system is a complex environment consisting of billions of neurons as well as glial support cells. These neurons use many different chemical signals to communicate information, including neurotransmitters, cannabinoids, peptides, and gases such as nitric oxide. Neuropeptides are secreted primarily from the central and peripheral nervous systems, exerting a broad spectrum of biological functions that includes the regulation of metabolism, reproduction and immunity.
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: < 15
  • Intra-assay: < 10
Panel TypeMAGNETIC Neuroscience
Quality LevelMQ200
Applications
ApplicationThe analytes available for this multiplex kit are: α-MSH, β-Endorphin, Neurotensin, Oxytocin, Substance P.
Key Applications
  • Multiplexing
Application Notes• This is an overnight assay. Note that this is a competitive assay.
• This assay requires 50 μL extracted serum or plasma sample per well or 50 μL neat CSF or culture media per well.
Biological Information
SpecificityThere was no or negligible cross-reactivity between the antibodies for an analyte and any of the other analytes in this panel.
Species Reactivity
  • Rat
  • Mouse
Analytes Available
  • α-MSH
  • β-Endorphin
  • Neurotensin
  • Oxytocin
  • Substance P
Accuracy
  • Rat: 107% - 135%
  • Mouse: 113% - 136%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitivities of individual biomarkers.
Standard Curve Range
  • α-MSH 41 – 30,000 pg/mL
  • β-Endorphine 69-50,000 pg/mL
  • Neurotensin 27-20,000 pg/mL
  • Oxytocin 14-100,000 pg/mL
  • Substance P 3-2,000 pg/mL
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Global Trade Item Number
Catalogue Number GTIN
RMNPMAG-83K 04053252988592