Our broad portfolio consists of multiplex panels that allow you to choose, within the panel, analytes that best meet your needs. On a separate tab you can choose the premixed cytokine format or a single plex kit.
Cell Signaling Kits & MAPmates™
Choose fixed kits that allow you to explore entire pathways or processes. Or design your own kits by choosing single plex MAPmates™, following the provided guidelines.
The following MAPmates™ should not be plexed together:
-MAPmates™ that require a different assay buffer
-Phospho-specific and total MAPmate™ pairs, e.g. total GSK3β and GSK3β (Ser 9)
-PanTyr and site-specific MAPmates™, e.g. Phospho-EGF Receptor and phospho-STAT1 (Tyr701)
-More than 1 phospho-MAPmate™ for a single target (Akt, STAT3)
-GAPDH and β-Tubulin cannot be plexed with kits or MAPmates™ containing panTyr
.
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To begin designing your MILLIPLEX® MAP kit select a species, a panel type or kit of interest.
Custom Premix Selecting "Custom Premix" option means that all of the beads you have chosen will be premixed in manufacturing before the kit is sent to you.
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Add Additional Reagents (Buffer and Detection Kit is required for use with MAPmates)
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48-602MAG
Buffer Detection Kit for Magnetic Beads
1 Kit
Space Saver Option Customers purchasing multiple kits may choose to save storage space by eliminating the kit packaging and receiving their multiplex assay components in plastic bags for more compact storage.
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MHSTCMAG-70KPMX
MilliporeMILLIPLEX MAP Mouse High Sensitivity T Cell Premixed Panel - Immunology Multiplex Assay
Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
More>>Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples. Less<<
MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
MILLIPLEX MAP Mouse High Sensitivity T Cell Premixed Panel - Immunology Multiplex Assay
Background Information
Cytokines are immunomodulatory polypeptides that play key roles in both adaptive and innate immune responses. A generic term, “cytokines” includes myokines (produced by muscle cells), lymphokines (produced by activated Th cells), interleukins (acting as mediators between T cells), and chemokines (responsible for T-cell migration). One of the regulatory mechanisms of the immune system, cytokines act at the recognition, activation, or effector phases of an immune response, modulating the development and functional activities of the subtypes of T cells, B cells, and myeloid cells. Research involving cytokines plays a significant role in achieving a deeper understanding of the immune system and its multi-faceted response to most antigens, especially those responses that make up the inflammatory process.
Low levels of inflammation are involved in many clinical and sub-clinical disease states, such as autoimmune disease, cardiovascular disease, diabetes, neurological disorders and cancer. Measuring picogram levels of cytokines, therefore, is critical for understanding the pathogenesis of these diseases.
Simultaneous analyze low levels of cytokine and chemokine biomarker with the High Sensitivity Bead-Based Multiplex Assays using the Luminex technology, in mouse serum, plasma and cell culture samples.
Key Applications
Multiplexing
Application Notes
• This is an overnight incubation assay. • A maximum of 25 μL per well of neat or 1:2 diluted serum or plasma can be used. Tissue culture or other media may also be used.
Biological Information
Specificity
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
Mouse
Analytes Available
GM-CSF
IFNγ
IL-1α
IL-1β
IL-2
IL-4
IL-5
IL-6
IL-7
IL-10
IL-12 (p70)
IL-13
IL-17A
KC/CXCL1
LIX
MCP-1
MIP-2
TNF-α
Cross Reactivity
There is no detectable cross-reactivity within the panel.
Accuracy
82-102%
Physicochemical Information
Sensitivity
0.26 - 11.14 pg/mL
Please see protocol for sensitivity of individual analytes.
Linearity of Dilution
Please contact technical service for information
Standard Curve Range
GM-CSF: 6.1 – 25,000 pg/mL
IFNγ: 0.49 – 2,000 pg/mL
IL-1α: 3.91 – 16,000 pg/mL
IL-1β: 3.91 – 16,000 pg/mL
IL-2: 0.98 – 4,000 pg/mL
IL-4: 0.10 – 400 pg/mL
IL-5: 0.98 – 4,000 pg/mL
IL-6: 0.98 – 4,000 pg/mL
IL-7: 0.49 – 2,000 pg/mL
IL-10: 1.34 – 5,500 pg/mL
IL-12 (p70): 2.93 – 12,000 pg/mL
IL-13: 3.42 – 14,000 pg/mL
IL-17A: 0.49 – 2,000 pg/mL
KC/GROα/CXCL1: 0.73 – 3,000 pg/mL
LIX: 9.77 – 40,000 pg/mL
MCP-1/CCL2: 3.17 – 13,000 pg/mLL
MIP-2/CXCL2: 7.32 – 30,000 pg/mL
TNFα: 0.49 – 2,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage Conditions
Recommended storage for kit components is 2 - 8°C.
