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344291 Freund’s Incomplete Adjuvant, Modified

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344291
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344291-10MLCN
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      Description
      OverviewContains 85% Drakeol 5NF, 15% Arlacel A (mannide monooleate emulsifier). Heat sterilized. Contains zero colony forming units/ml.
      Catalogue Number344291
      Brand Family Calbiochem®
      References
      ReferencesFreund, J. 1947. Ann. Rev. Microbiol. 1, 291.
      Product Information
      FormYellow oily liquid
      Quality LevelMQ100
      Applications
      ApplicationFreund’s Incomplete Adjuvant, Modified Contains 85% Drakeol 5NF, 15% Arlacel A. Heat sterilized. Contains zero colony forming units.
      Application Comments10 ml of Freund's Incomplete Adjuvant homogenized in the following amounts by weight:

      85% Drakeol 5 NF (light mineral oil)
      15% Arlacel A, mannide monooleate emulsifier.

      1. Take the vial of Friend's Incomplete Adjuvant and fold the aluminum seal back to permit free access to the stopper. Be careful not to break the aluminum ring that holds the stopper in place.
      2. Dissolve 50 mg of antigen in 5.0 ml of sterile PBS, pH 7.0-7.3. Maintain or create sterility if at all feasible.
      3. Draw the solution into a 10 ml syringe with a 1 inch 21 gauge needle.
      4. Insert needle into adjuvant vial and forcibly drive solution into the vial. Withdraw about half the mixture into syringe and force it into the adjuvant vial again.
      5. Repeat the mixing procedure in step 4 until a smooth, stable, milky, water-in-oil emulsion forms. Check if the emulsion is complete by expelling a drop from the syringe onto the water surface in a beaker. If the drop remains on the surface and does not disperse when the beaker is agitated slightly, the emulsion is adequate. A properly prepared emulsion is stable for days.
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +15°C to +30°C
      Do not freeze Ok to freeze
      Global Trade Item Number
      Catalogue Number GTIN
      344291-10MLCN 04055977214703