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324727 Endoglycosidase F3, Elizabethkingia meningosepticum, Recombinant, E. coli

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324727
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324727-200MIUCN
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      Description
      OverviewRecombinant, Elizabethkingia meningosepticum endoglycosidase F3 expressed in E. coli. Cleaves asparagine-linked or free biantennary and triantennary complex, and Man3GlcNAc oligosaccharides from glycoproteins. This enzyme cleaves between the two N-acetylglucosamine residues in the diacetylchitobiose core of the oligosaccharide, generating a truncated sugar molecule with one N-acetylglucosamine residue remaining on the asparagine residue. Core fucosylation increases the activity of Endo F3 up to 40 fold. Exhibits no activity on high mannose and hybrid molecules. Less sensitive to protein conformation than N-Glycosidase F (Cat. No. 362185) and therefore is more suitable for deglycosylation of native proteins.
      Note: 1 mU = 1 milliunit.
      Catalogue Number324727
      Brand Family Calbiochem®
      SynonymsEndo-β-N-acetylglucosaminidase F3, Endo F3
      References
      ReferencesTarentino, A.L., et al. 1995. Glycobiology 5, 599.
      Tarentino, A.L., and Plummer, T.H. 1994. Methods Enzymol. 230, 44.
      Tarentino, A.L., et al. 1993. J. Biol. Chem. 268, 9702.
      Trimble, R.B., and Tarentino, A.L. 1991. J. Biol. Chem. 266, 1646.
      Product Information
      Activity≥5 units/ml
      Unit of DefinitionOne unit is defined as the amount of enzyme that will release N-linked oligosaccharides from 1.0 µmol porcine fibrinogen per min at 37°C, pH 5.5.
      EC number3.2.1.96
      FormLiquid
      FormulationIn 20 mM Tris-HCl, pH 7.5.
      Quality LevelMQ100
      Biological Information
      Specific Activity≥30 units/mg protein
      Physicochemical Information
      ContaminantsProteases: none detected
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Yes
      Global Trade Item Number
      Catalogue Number GTIN
      324727-200MIUCN 04055977216011