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Merck

1.00898

E.coli / Coliform Selective-Supplement

for Escherichia coli, for coliforms, pkg of 10 vials, for use with ChromoCult® Coliform Agar

Synonym(s):

E. coli / Coliform supplement, E. coli / Coliform antibiotic Selective Supplement

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About This Item

NACRES:
NA.74
UNSPSC Code:
41106212

Key Documents

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Quality Level

200

compatibility

for use with ChromoCult® Coliform Agar, (1.10426)

storage temp.

2-8°C

suitability

Escherichia coli, coliforms

form

lyophilized powder

composition

Cefsulodine, 2.5 mg per vial , Vancomycin, 2.5 mg per vial

packaging

pkg of 10 vials

technique(s)

microbiological culture: suitable

pH

3.5-4.5 (25 °C, 14 g/L in H2O)

application(s)

food and beverages
microbiology

Related Categories

General description

E. coli / Coliform supplement is a mixture of two antibiotics in lyophilized form. Vancomycin inhibits the growth of Gram-positive bacteria. Cefsulodin suppresses the growth of Pseudomonas spp. and Aeromonas spp. This selective supplement can be used in combination with most E. coli / Coliform enrichment media and plating agars.

Application

E. coli / Coliform Selective-Supplement is an additive for the preparation of selective culture media for the detection of E. coli / Coliforms in water, food, and environmental samples.

Analysis Note

Supplement tested in Chromocult Coliform agar art. no. 1.10426.


Recovery on test medium


- Escherichia coli DSM 502:


≥ 70 %


- Citrobacter freundii ATCC 8090: ≥ 70 %


Growth


- Aeromonas hydrophila ATCC 7966 (WDCM 00063):


inhibited


- Pseudomonas aeruginosa ATCC 27853 (WDCM 00025): inhibited


- Bacillus licheniformis ATCC 14580 (WDCM 00068): inhibited


- Staphylococcus aureus ATCC 6538 (WDCM 00032): inhibited


Colony colour


- Escherichia coli DSM 502:


blue


- Citrobacter freundii ATCC 8090: red


Incubation: 24 hrs.; 35 °C; aerobic.

Other Notes

Replaces: 1.00898

Legal Information

CHROMOCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

pictograms

Health hazard
GHS08

signalword

Danger

Hazard Classifications

Resp. Sens. 1 - Skin Sens. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

Certificates of Analysis (COA)

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Global Trade Item Number

SKUGTIN
1.00898.001004027269168605

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