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428901 Cholesterol/Cholesteryl Ester Quantitation Kit

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428901
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428901-1KITCN
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      Description
      OverviewA sensitive assay for the quantitative measurement of cholesterol and/or cholesteryl ester by a colorimetric or a fluorometric method. Measurement by spectrophotometry at OD =570 nm or fluorometry at Ex/Em =535/587 nm. This assay can measure cholesterol itself, total cholesterol (cholesterol + cholesterol ester) by adding cholesterol esterase to the reaction, or only cholesteryl ester by subtracting the value of cholesterol from the total value of cholesterol plus cholesteryl esters. The fluorometric assay can detect 0.02-1 µg/well and is 4-10 fold more sensitive than colorimetric assay. Kit contains sufficient reagents for 100 tests.
      Catalogue Number428901
      Brand Family Calbiochem®
      Materials Required but Not Delivered Spectrophotometer capable of reading absorbance at 570 nm or a fluorimeter capable of measuring fluorescence at an excitation wavelength of ~535 nm and an emission wavelength of ~587 nm
      96-well plate
      Chloroform-NP-40 (1% NP-40 in pure chloroform) (for preparation of cell lysates and tissue extracts)
      Product Information
      Form100 Tests
      Format96-well plate
      Kit containsCholesterol Reaction Buffer, Cholesterol Probe, Dimethylsulfoxide, Enzyme Mix, Cholesterol Esterase, Cholesterol Standard, and a user protocol.
      Quality LevelMQ100
      Biological Information
      Assay range0.2-10 µg/well
      Sample TypeCells, tissues, serum
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Multiple Toxicity Values, refer to MSDS
      Storage -20°C
      Storage ConditionsUpon arrival store the entire contents of the kit at -20°C.
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Supplemental Information
      Kit containsCholesterol Reaction Buffer, Cholesterol Probe, Dimethylsulfoxide, Enzyme Mix, Cholesterol Esterase, Cholesterol Standard, and a user protocol.
      Global Trade Item Number
      Catalogue Number GTIN
      428901-1KITCN 04055977210118