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MABC1612 Anti-phospho-PHD2 (Ser 125) Antibody, clone 4

Anti-phospho-PHD2 (Ser 125) clone 4, Cat. No. MABC1612, is a mouse monoclonal antibody that detects PHD2 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: EGLN1, C1orf12, PNAS-118, PNAS-137 UniProt Number: Q9GZT9
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MABC1612
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, IF, IH(P), IPMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABC1612
      DescriptionAnti-phospho-PHD2 (Ser 125) Antibody, clone 4
      Alternate Names
      • Egl nine homolog 1
      • EC:1.14.11.29
      • Hypoxia-inducible factor prolyl hydroxylase 2
      • HIF-PH2
      • HIF-prolyl hydroxylase 2
      • HPH-2
      • Prolyl hydroxylase domain-containing protein 2
      • SM-20
      Background InformationEgl nine homolog 1 (UniProt: Q9GZT9; also known as EC:1.14.11.29, Hypoxia-inducible factor prolyl hydroxylase 2, HIF-PH2, HIF-prolyl hydroxylase 2, HPH-2, Prolyl hydroxylase domain-containing protein 2, PHD2, SM-20) is encoded by the EGLN1 (also known as C1orf12, PNAS-118, PNAS-137) gene (GeneID: 54583) in human. PHD2 acts as a cellular oxygen sensor that catalyzes, under normoxic conditions, the post-translational formation of 4-hydroxyproline in hypoxia-inducible factor (HIF) alpha proteins. It is widely expressed with highest levels in skeletal muscle and heart, moderate levels in pancreas, brain and kidney, and lower levels in lung and liver. PHD2 is mainly cytoplasmic, but it shuttles between the nucleus and cytoplasm. It hydroxylates a specific proline found in each of the oxygen-dependent degradation (ODD) domains (N-terminal, NODD, and C-terminal, CODD) of HIF1alpha and is also reported to hydroxylate HIF2alpha. PHD2 is reported to be phosphorylated on serine 125 by p70S6K and this phosphorylation increased its ability to degrade HIF1alpha. PHD2 exhibits a preference for the CODD site for both HIF1alpha and HIF1beta. Hydroxylated HIFs are then targeted for proteasomal degradation via the von Hippel-Lindau ubiquitination complex. Under hypoxic conditions, the hydroxylation reaction is attenuated allowing HIFs to escape degradation resulting in their translocation to the nucleus and heterodimerization with HIF1beta. It is one of the most important isozyme under normoxia and, through regulating the stability of HIF1, it is involved in various hypoxia-influenced processes such as angiogenesis in retinal and cardiac functionality. Defects in EGLN1 gene cause familial type erythrocytosis, an autosomal dominant disorder that is characterized by increased serum red blood cell mass, elevated serum hemoglobin and hematocrit, and normal serum erythropoietin levels. (Ref.: Di Conza, G., et al. (2017). Cell Rep. 18(7): 1699-1712).
      References
      Product Information
      FormatPurified
      PresentationPurified mouse monoclonal antibody IgG2a in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-PHD2 (Ser 125) clone 4, Cat. No. MABC1612, is a mouse monoclonal antibody that detects PHD2 and has been tested for use in Immunofluorescence, Immunohistochemistry (Paraffin), Immunoprecipitation, and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunofluorescence
      • Immunohistochemistry (Paraffin)
      • Immunoprecipitation
      Application Notes.
      Biological Information
      ImmunogenA linear peptide corresponding to 11 amino acids surrounding Ser125 phosphorylation site from the N-terminal half of human PHD2.
      Clone4
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone 4 detects PHD2 in lysate of HEK293 cells overexpressing PHD2.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • EGLN1
      • C1orf12
      • PNAS-118
      • PNAS-137
      Modifications
      • Phosphorylation
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~50 kDa observed; 46.02 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in lysates of HEK293 cells overexpressing PHD2.

      Western Blotting Analysis: 1 µg/mL of this antibody detected phospho-PHD2 (Ser 125) in 10 µg of lysate from HEK293 cells overexpressing PHD2.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABC1612 04054839243523