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OP16L Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4)

Anti-c-ErbB2/c-Neu (Ab-4), mouse monoclonal, clone 7.16.4, recognizes the ~185 kDa c-ErbB2/c-Neu in overexpressing B104-1-1 cells. It is validated for WB, ICC, IP, and IHC with frozen sections.

Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: Anti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus

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OP16L
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Overview

Replacement Information

Key Spec Table

Species ReactivityHostAntibody Type
H, RMMonoclonal Antibody

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      Description
      OverviewRecognizes the ~185 kDa c-ErbB2/c-Neu protein in overexpressing B104-1-1 cells.
      Catalogue NumberOP16L
      Brand Family Calbiochem®
      SynonymsAnti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
      References
      ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
      Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
      Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
      Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
      Slamon, D.J., et al. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319, 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Product Information
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      Negative controlFisher rat embryo cells
      Positive controlB104-1-1 cells
      PreservativeNone
      Quality LevelMQ100
      Applications
      Application ReferencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Growth Inhibition Assays Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.
      Key Applications Frozen Sections
      Growth Inhibition Assay
      Immunoblotting (Western Blotting)
      Immunocytochemistry
      Immunoprecipitation
      Application NotesGrowth Inhibition Assays (see application references)
      Frozen Sections (5 µg/ml, see application references)
      Immunoblotting (see application references)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (1 µg/ml, see application references)
      Application CommentsStains frozen tissue sections of rat cells overexpressing the c-neu protein. This antibody does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
      ImmunogenHuman
      Epitopewithin the extracellular domain
      Clone7.16.4
      HostMouse
      IsotypeIgG2a
      Species Reactivity
      • Human
      • Rat
      Antibody TypeMonoclonal Antibody
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage +2°C to +8°C
      Do not freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      OP16L-100UGCN 04055977208733

      Documentation

      Anti-c-ErbB2/c-Neu (Ab-4) Mouse mAb (7.16.4) Certificates of Analysis

      TitleLot Number
      OP16L

      References

      Reference overview
      Zhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
      Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
      Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
      Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
      Slamon, D.J., et al. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319, 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.

      Citations

      Title
    • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
      		•
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-November-2007 JSW
      SynonymsAnti-HER2, Anti-Neu, Anti-ErbB2, Anti-Erythroblastosis Virus
      ApplicationGrowth Inhibition Assays (see application references)
      Frozen Sections (5 µg/ml, see application references)
      Immunoblotting (see application references)
      Immunocytochemistry (5 µg/ml)
      Immunoprecipitation (1 µg/ml, see application references)
      DescriptionPurified mouse monoclonal antibody generated by immunizing C3H mice with the specified immunogen and fusing splenocytes with NS-1 mouse myeloma cells (see application references). Recognizes the ~185 kDa c-Erb2/c-neu protein.
      BackgroundAmplification of the human proto-oncogene c-neu occurs in approximately 30% of breast carcinomas and the amplification maybe a useful predictor of disease prognosis. Gene amplification and the resulting overexpression of proto-oncogene encoded proteins is thought to transform cells by chronically stimulating signal transduction pathways and in model systems overexpression of human c-neu induces cellular transformation. In the rat model system, a point mutation has been located within the transmembrane domain of the c-neu oncogene. neu (from neuroglioblastomas) is also referred to as erbB-2 (from erythroblastosis virus) and HER2 (for Human Epidermal Growth Factor Receptor) and was the second of four members of the EGF receptor family to be described.
      HostMouse
      Immunogen speciesHuman
      ImmunogenB104-1-1 cells overexpressing rat c-ErbB2/c-Neu
      Epitopewithin the extracellular domain
      Clone7.16.4
      IsotypeIgG2a
      Specieshuman, rat
      Positive controlB104-1-1 cells
      Negative controlFisher rat embryo cells
      FormLyophilized
      FormulationLyophilized from a volatile buffer, 100 µg BSA.
      PreservativeNone
      CommentsStains frozen tissue sections of rat cells overexpressing the c-neu protein. This antibody does not inhibit in vitro protein-tyrosine kinase activity, but it will inhibit in vivo growth of tumors overexpressing the neu oncogene (see application references). Does not cross-react with the EGF receptor. Antibody should be titrated for optimal results in individual systems.
      Storage +2°C to +8°C
      Do Not Freeze Ok to freeze
      Special InstructionsReconstitute the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Lyophilized antibodies should be reconstituted at 4°C with occasional gentle mixing for at least 2 h. Following reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage with 0.1% azide. Avoid freeze/thaw cycles of solutions.
      Toxicity Standard Handling
      ReferencesZhang, H., et al. 1999. Exp. Mol. Pathol. 67, 15.
      Peterson, N.C. and Greene, M.I., 1988. DNA Cell Biol. 17, 1031.
      Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389.
      Van De Vijver, M.J., et al. 1988. New Engl. J. Med. 319, 1239.
      DiFiore, P.P., et al. 1987. Science 237, 178.
      Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498.
      Slamon, D.J., et al. Science 235, 177.
      Varley, J.M., et al. 1987. Oncogene 1, 423.
      Bargmann, C.I., et al. 1986. Nature 319, 226.
      Yamamoto, T., et al. 1986. Nature 319, 230.
      Blick, M., et al. 1984. Blood 64, 1234.
      Schwab, M., et al. 1984. Cold Spring Harbor Laboratory 2, 215.
      Citation
    • R. Bruce Montgomery, et al. (2005) Endogenous Anti-HER2 Antibodies Block HER2 Phosphorylation and Signaling through Extracellular Signal-Regulated Kinase. Cancer Research 65, 650-656.
      		•
      Application referencesImmunoprecipitation Kokai, Y., et al. 1989. Cell 58, 287. Kokai, Y., et al. 1988. Proc. Natl. Acad. Sci. USA 85, 5389. Frozen Sections Kokai, Y., et al. 1987. Proc. Natl. Acad. Sci. USA 84, 8498. Original Clone, Growth Inhibition Assays Drebin, J.A., et al. 1988. Oncogene 2, 273. Immunoblotting Montgomery, R.B., et al. 2005. Cancer Res. 65, 650.

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies